Method for sterilizing membrane comprising glucose oxidase and associated bio-sensor

Abstract

A method for sterilizing a membrane comprising an oxidoreductase enzyme, comprises: irradiating with gamma radiation the membrane comprising an oxidoreductase enzyme soaked in an aqueous buffer solution. Associated biosensors and bioreactors are also described.

1 . A method for sterilizing a membrane comprising an oxidoreductase enzyme, comprising: irradiating with gamma radiation the membrane comprising an oxidoreductase enzyme soaked in an aqueous buffer solution. 
     
     
         2 . The method of  claim 1 , wherein the oxidoreductase enzyme is selected from the group consisting of glucose oxidase, lactate oxidase and glutamate oxidase. 
     
     
         3 . The method of  claim 2 , wherein the oxidoreductase enzyme is glucose oxidase. 
     
     
         4 . The method of  claim 1 , wherein the aqueous buffer solution is a phosphate buffer. 
     
     
         5 . The method of  claim 4 , wherein the aqueous buffer solution is a phosphate buffer saline (PBS) solution. 
     
     
         6 . The method of  claim 1 , wherein the aqueous buffer solution is substantially free from organic components. 
     
     
         7 . A method for sterilizing a membrane comprising glucose oxidase, comprising: irradiating with gamma radiation the membrane comprising glucose oxidase soaked in a phosphate buffer saline (PBS) solution. 
     
     
         8 . The method of  claim 1 , wherein a dose of the gamma radiation is in a range of from about 25 kGy to about 40 kGy. 
     
     
         9 . The method of  claim 1 , wherein a rate of the gamma radiation is about 20 kGy/hour. 
     
     
         10 . The method of  claim 1 , wherein the membrane is in a plastic container. 
     
     
         11 . The method of  claim 1 , wherein the membrane is in a biosensor. 
     
     
         12 . The method of  claim 11 , wherein the biosensor comprises a Pt electrode and an Ag/AgCl electrode. 
     
     
         13 . A biosensor comprising the membrane sterilized using the method of  claim 1 . 
     
     
         14 . The biosensor of  claim 13 , wherein the membrane comprises glucose oxidase and the sensitivity of the biosensor to glucose is about 80% of the sensitivity of a biosensor comprising a membrane comprising glucose oxidase without sterilization to glucose. 
     
     
         15 . The biosensor of  claim 13 , comprising a Pt electrode and an Ag/AgCl electrode. 
     
     
         16 . A biosensor comprising a membrane comprising glucose oxidase, sterilized by: irradiating with gamma radiation while the membrane comprising glucose oxidase is soaked in a phosphate buffer saline (PBS) solution. 
     
     
         17 . A bioreactor comprising the biosensor of  claim 13 .