US2015166971A1PendingUtilityA1

Polypeptides Having Cellulase Activity and Polynucleotides Encoding Same

44
Assignee: NOVOZYMES ASPriority: Aug 15, 2011Filed: Feb 26, 2015Published: Jun 18, 2015
Est. expiryAug 15, 2031(~5.1 yrs left)· nominal 20-yr term from priority
C12N 9/2437C11D 3/38645
44
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Claims

Abstract

The present invention relates to isolated polypeptides having cellulase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Claims

exact text as granted — not AI-modified
1 - 15 . (canceled) 
     
     
         16 . An isolated polypeptide having cellulase activity, selected from the group consisting of:
 (a) a polypeptide having at least 60% sequence identity to the mature polypeptide of SEQ ID NO:2;   (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions with the mature polypeptide coding sequence of SEQ ID NO:1, or the full-length complement thereof;   (c) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:1 or the genomic DNA sequence thereof; or   (d) a variant of the mature polypeptide of SEQ ID NO:2 comprising a substitution, deletion, and/or insertion at one or more (e.g., several) positions; and   (e) a fragment of the polypeptide of (a), (b) or (c) that has cellulase activity.   
     
     
         17 . A composition comprising the polypeptide of  claim 16 . 
     
     
         18 . The composition of  claim 17 , which is a detergent composition. 
     
     
         19 . An isolated polynucleotide encoding the polypeptide of  claim 16 . 
     
     
         20 . A nucleic acid construct or expression vector comprising the polynucleotide of  claim 19  operably linked to one or more control sequences that direct the production of the polypeptide in an expression host. 
     
     
         21 . A recombinant host cell comprising the polynucleotide of  claim 19  operably linked to one or more control sequences that direct the production of the polypeptide. 
     
     
         22 . A method of producing a polypeptide having cellulase activity, comprising:
 (a) cultivating the host cell of  claim 21  under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.   
     
     
         23 . A method of cleaning a fabric, comprising treating the fabric with a polypeptide of  claim 16  in a wash liquor.

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