Process for Preparation of a Grifola frondosa Polysaccharide F2 and Its Hypoglycemic Activity

Abstract

A Grifola frondosa polysaccharide F2 with hypoglycemic activity, process for preparation and use thereof. The process for preparation of Grifola frondosa polysaccharide F2 is as follows: The fruit bodies of Grifola frondosa were homogenized to a fine powder and extracted with hot water. The mixture was filtered and precipitated with absolute ethanol. The precipitation was obtained. The said precipitation was applied on DEAE Sepharose Fast chromatographic column, equilibrated with Tris-HCl(10 mM, pH=8.0), collecting the efficient eluting peak to obtain the fraction F1; eluted with Tris-HCl(10 mM, pH=8.0) which contains 0.1M NaCl, fraction F2 was obtained; then concentrated under reduced pressure, dialyzed and lyophilized, Grifola frondosa polysaccharide F2 was obtained. This isolates a new Grifola frondosa polysaccharide F2 with hypoglycemic activity from the fruit bodies of Grifola frondosa. The Grifola frondosa polysaccharide F2 can be used in manufacturing a drug for treating diabetes. The polysaccharide makes a foundation for developing new anti-diabetes agents.

1 . A process for preparation of  Grifola frondosa  polysaccharide F2, comprising:
 (a) the fruit bodies of  Grifola frondosa  are homogenized to a fine powder and extracted with hot water; the mixture was filtered and precipitated with absolute ethanol; the precipitation was obtained.   (b) the said precipitation is applied on DEAE Sepharose Fast chromatographic column, equilibrated with Tris-HCl(10 mM, pH=8.0), collecting the efficient eluting peak to obtain the fraction F1; eluted with Tris-HCl(10 mM, pH=8.0) which contains 0.1M NaCl, fraction F2 was obtained; then concentrated under reduced pressure, dialyzed and lyophilized,  Grifola frondosa  polysaccharide F2 is obtained.   
     
     
         2 . A process for preparation of  Grifola frondosa  polysaccharide F2 according to  claim 1 , characterized in that the said precipitation in the step (a) is dissolved into hot water, filtered by a 0.45 μm millipore filter and dialyzed in dialysis bag of 3000 D for 24 h to yield crude polysaccharides; the concentration of crude polysaccharide in the  Grifola frondosa  crude polysaccharides solution was adjusted, and then be used as the sample of chromatographic separation in the step (b). 
     
     
         3 .  Grifola frondosa  polysaccharide F2 prepared by the process for preparation of  Grifola frondosa  polysaccharide F2 as claimed in  claim 1 . 
     
     
         4 . Use of  Grifola frondosa  polysaccharide F2 as claimed in  claim 3  for manufacturing a drug for the treatment of diabetes. 
     
     
         5 . The use according to  claim 4 , wherein, the said diabetes is type 2 diabetes. 
     
     
         6 . A drug for the treatment of diabetes, characterized in that the said medicament comprises  Grifola frondosa  polysaccharide F2 as claimed in  claim 3  as active ingredients. 
     
     
         7 . The drug according to  claim 6 , wherein the said diabetes is type 2 diabetes.