Agent and kit for isolating nucleic acids

Abstract

A method for isolating nucleic acids is provided. The method includes providing a biological sample containing at least one nucleic acid, and mixing the biological sample with an isolating agent under a suitable condition to isolate the nucleic acids from the biological sample in single step, wherein the isolating agent contains 1-40 wt % of PEG and/or more than 30 wt % of low molecular weight alcohol, a salt, and a detergent. Isolated nucleic acids are bound to a solid support by changes in the solubility of nucleic acids. Additionally, the present invention further provides an isolating agent and kit for isolating nucleic acids.

What is claimed is: 
     
         1 . An isolating agent, comprising 1-40 wt % of PEG and/or more than 30 wt % of low molecular weight alcohol, a salt, and a detergent, and a solid support. 
     
     
         2 . The isolating agent as claimed in  claim 1 , wherein the low molecular weight alcohol has a molecular weight of 30-100 g/mol. 
     
     
         3 . The isolating agent as claimed in  claim 1 , wherein the low molecular weight alcohol is ethanol. 
     
     
         4 . The isolating agent as claimed in  claim 1 , wherein the low molecular weight alcohol has a concentration of 40-60 wt %. 
     
     
         5 . The isolating agent as claimed in  claim 1 , wherein the PEG has a concentration of 5-20 wt %. 
     
     
         6 . The isolating agent as claimed in  claim 1 , wherein the salt comprises an alkali halide, an alkaline earth halide, and/or an ammonium halide. 
     
     
         7 . The isolating agent as claimed in  claim 1 , wherein the detergent comprises Tween-20, Triton X-100, NP40, Brij35, or CHAPS. 
     
     
         8 . The isolating agent as claimed in  claim 1 , wherein the solid support comprises a magnetic material, a metal, a metal oxide, a silicide, or a polymer. 
     
     
         9 . The isolating agent as claimed in  claim 1 , wherein the isolating agent further comprises a protease. 
     
     
         10 . The isolating agent as claimed in  claim 1 , wherein the isolating agent further comprises a saccharide. 
     
     
         11 . The isolating agent as claimed in  claim 10 , wherein the saccharide comprises a monosaccharide or polysaccharide derivative. 
     
     
         12 . The isolating agent as claimed in  claim 11 , wherein the polysaccharide comprises a glycogen, a starch, a oligosaccharides, a dextran, a cellulose, a agarose, a chitosan, a mucopolysaccharide, or a peptidoglycan. 
     
     
         13 . A kit for isolating nucleic acids, comprising:
 an isolating agent, comprising 1-40 wt % of PEG and/or more than 30 wt % of low molecular weight alcohol, a salt, and a detergent, and   a container.   
     
     
         14 . The kit as claimed in  claim 13 , wherein the low molecular weight alcohol has a molecular weight of 30-100 g/mol. 
     
     
         15 . The kit as claimed in  claim 13 , wherein the low molecular weight alcohol is ethanol. 
     
     
         16 . The kit as claimed in  claim 13 , wherein the low molecular weight alcohol has a concentration of 40-60 wt %. 
     
     
         17 . The kit as claimed in  claim 13 , wherein the PEG has a concentration of 5-20 wt %. 
     
     
         18 . The kit as claimed in  claim 13 , wherein the solid support comprises a magnetic material, a metal, a metal oxide, a silicide, or a polymer. 
     
     
         19 . The kit as claimed in  claim 13 , wherein the salt comprises an alkali halide, an alkaline earth halide, and/or an ammonium halide. 
     
     
         20 . The kit as claimed in  claim 13 , wherein the detergent comprises Tween-20, Triton X-100, NP40, Brij35, or CHAPS. 
     
     
         21 . The kit as claimed in  claim 13 , wherein the isolating agent further comprises a protease. 
     
     
         22 . The kit as claimed in  claim 13 , wherein the isolating agent further comprises a saccharide. 
     
     
         23 . The kit as claimed in  claim 22 , wherein the saccharide comprises a monosaccharide or polysaccharide derivative. 
     
     
         24 . The kit as claimed in  claim 23 , wherein the polysaccharide comprises a glycogen, a starch, a oligosaccharides, a dextran, a cellulose, a agarose, a chitosan, a mucopolysaccharide, or a peptidoglycan. 
     
     
         25 . The kit as claimed in  claim 13 , further comprises a wash solution and/or elution solution. 
     
     
         26 . The kit as claimed in  claim 25 , wherein the wash solution removes salts and proteins. 
     
     
         27 . The kit as claimed in  claim 25 , wherein the elution solution elutes nucleic acids from the solid support. 
     
     
         28 . The kit as claimed in  claim 13 , wherein the container packs the isolating agent.