Adipose-derived stem cells and lattices

Abstract

The present invention provides adipose-derived stem cells and lattices. In one aspect, the present invention provides a lipo-derived stem cell substantially free of adipocytes and red blood cells and clonal populations of connective tissue stem cells. The cells can be employed, alone or within biologically-compatible compositions, to generate differentiated tissues and structures, both in vivo and in vitro. Additionally, the cells can be expanded and cultured to produce hormones and to provide conditioned culture media for supporting the growth and expansion of other cell populations. In another aspect, the present invention provides a lipo-derived lattice substantially devoid of cells, which includes extracellular matrix material from adipose tissue. The lattice can be used as a substrate to facilitate the growth and differentiation of cells, whether in vivo or in vitro, into anlagen or even mature tissues or structures.

1 . A clonally isolated mammalian lipo-derived stem cell substantially free of mature adipocytes which differentiates into an ectodermal and/or endodermal phenotypes and any of adipogenic, chondrogenic, cardiogenic, dermatogenic, hematopoetic, hemangiogenic, myogenic, nephrogenic, urogenitogenic, osteogenic, pericardiogenic, peritoneogenic, and stromal developmental phenotypes. 
     
     
         2 - 79 . (canceled) 
     
     
         80 . A clonally isolated adipose derived stem cell. 
     
     
         81 . The cell of  claim 80 , which is human. 
     
     
         82 . The cell of  claim 80 , which is genetically modified. 
     
     
         83 . The cell of  claim 80 , which secretes a hormone. 
     
     
         84 . A cell population comprising a cell of  claim 80 . 
     
     
         85 . An isolated, human adipose-derived multipotent cell that differentiates into cells of two or more mesodermal phenotypes. 
     
     
         86 . A cell population comprising a cell of  claim 81 . 
     
     
         87 . A cell population comprising a cell of  claim 82 . 
     
     
         88 . A cell population comprising a cell of  claim 83 . 
     
     
         89 . The isolated, human adipose-derived multipotent cell of  claim 84 , wherein the cell has been passaged at least 10 times in culture. 
     
     
         90 . The isolated, human adipose-derived multipotent cell of  claim 84 , wherein the cell has been passaged at least 15 times in culture. 
     
     
         91 . The isolated, human adipose-derived multipotent cell of  claim 84 , wherein the cell has been passaged at least 20 times in culture.