Conversion of prenyl derivatives to isoprene

Abstract

The present invention provides methods for producing derivatives from cultured cells. In addition, the present invention provides methods for conversion of prenyl deerivatives, obtained from biological or petrochemical sources, to isoprene by employing chemical or biological catalysts. The present invention also provides compositions that include the cultured cells or isoprene or prenyl derivatives produced there from.

1 - 9 . (canceled) 
     
     
         10 . A method of producing isoprene, the method comprising
 (a) culturing host cells comprising one or more nucleic acid(s) encoding one or more phosphatases of enzyme class 3.6.1, 3.1.7 or 3.1.3 under suitable culture conditions for the production of a prenyl derivative of Formula (II):   wherein R 3  is hydrogen or —C(O)R 4 ; and   R 4  is C 1 -C 5  linear or branched alkyl;   (b) recovering said prenyl derivative; and   (c) dehydrating or decarboxylating said prenyl derivative to produce isoprene.   
     
     
         11 . The method of  claim 10 , wherein the prenyl derivative is recovered from a fermentation of the cultured cells using one or more processes selected from the group consisting of distillation, gas-stripping, two-phase recovery, and pervaporation. 
     
     
         12 . The method of  claim 10 , wherein the prenyl derivative comprises 2-methyl-3-buten-2-ol. 
     
     
         13 . The method of  claim 10 , wherein the cells further comprise one or more of an isopentenyl-diphosphate-delta-isomerase (IDI) polypeptide, one or more mevalonic acid (MVA) pathway polypeptides, and/or one or more deoxyxylulose-5-phosphate (DXP) pathway polypeptides. 
     
     
         14 . The method of  claim 10 , wherein the cells further comprise one or more lower MVA pathway polypeptides. 
     
     
         15 . The method of  claim 10 , wherein the one or more phosphatases of enzyme class 3.6.1, 3.1.7 or 3.1.3 are one or more phosphatases selected from the group consisting of an allyl diphosphatase, an ADP-ribose pyrophosphatase, an ADP-sugar phophorylase, a nucleoside triphosphate pyrophosphatase, a FAD pyrophosphatase, a monoterpenyl pyrophosphastase, an alkaline phosphatase, and an acid phosphatase. 
     
     
         16 . (canceled) 
     
     
         17 . The method of  claim 10 , wherein the dehydration or decarboxylation reaction of step (c) comprises contacting the compound of Formula (I) with an inorganic or organic catalyst. 
     
     
         18 . The method of  claim 17 , wherein the inorganic catalyst is selected from the group consisting of HCl, HBr, H 2 SO 4 , H 3 P0 4 , a solid acid catalyst, an inorganic acid on an inert carrier, an activated alumina, and a zeolite. 
     
     
         19 . The method of  claim 17 , wherein the organic catalyst is selected from the group consisting of p-toluenesulfonic acid, trifluorornethane sulfonic acid, an organic acid resin, a Nafion, and a fluorosulfonic acid resin.