Cellulase-producing novel strain and saccharification method using the same

Abstract

The present invention relates to the novel strain Pholiota adiposa SKU714, a method for producing cellulase from the strain and a method for saccharifying cellulose using the produced cellulase. Since the cellulase produced by the novel strain according to the present invention exhibits better saccharification yield than the existing saccharification enzymes, it can be used in various applications, including bioenergy production, textile industry, papermaking industry, detergent industry, feed industry, food industry, production of low-calorie foods, fermentation of food wastes, or the like.

1 . A cellulase-producing  Pholiota adiposa  SKU714 deposited under Accession No. KCCM 11187P. 
     
     
         2 . A method for producing cellulase, comprising producing cellulase by culturing the  Pholiota adiposa  SKU714 according to  claim 1 . 
     
     
         3 . The method for producing cellulase according to  claim 2 , wherein the culturing is performed in a medium of pH 4.5-5.5 containing corn steep powder (5-10 g/L), yeast extract (1-5 g/L), potassium dihydrogen phosphate (3-7 g/L), potassium hydrogen phosphate (3-7 g/L), magnesium sulfate heptahydrate (1-5 g/L), thiamine hydrochloride (0.01-0.03 g/L) and a carbon source (10-30 g/L). 
     
     
         4 . The method for producing cellulase according to  claim 3 , wherein the carbon source is selected from a group consisting of cellulose, cellobiose, rice straw and avicel. 
     
     
         5 . The method for producing cellulase according to  claim 2 , wherein the culturing is performed under the condition of a stirring rate of 100-200 rpm, an aeration rate of 0.8-1.2 vvm and a culturing temperature of 25-30° C. 
     
     
         6 . A method for saccharifying cellulose, comprising saccharifying a cellulose substrate using cellulase produced by culturing the  Pholiota adiposa  SKU714 according to  claim 1 . 
     
     
         7 . The method for saccharifying cellulose according to  claim 6 , wherein the cellulose substrate is poplar, rice straw or a mixture thereof. 
     
     
         8 . The method for saccharifying cellulose according to  claim 6 , wherein the saccharification is performed under the condition of a substrate concentration of 5-25 wt %, a cellulase concentration of 1-45 FPU/g substrate, pH 4-7 and a temperature of 50-80° C. 
     
     
         9 . The method for producing cellulase according to  claim 3 , wherein the culturing is performed under the condition of a stirring rate of 100-200 rpm, an aeration rate of 0.8-1.2 vvm and a culturing temperature of 25-30° C.