Methods and compositions for plant pest control

Abstract

The present invention is directed to controlling nematode infestation. The invention discloses methods and compositions for use in controlling nematode infestation by providing recombinant DNA molecules to the cells of a plant in order to achieve a reduction in nematode infestation. The invention is also directed to methods for making transgenic plants that express the recombinant DNA molecule for use in protecting plants from nematode infestation.

1 . A polynucleotide comprising a sequence encoding a polypeptide comprising a plant methylketone synthase and a heterologous transit peptide operably linked to the plant methylketone synthase, wherein the plant methylketone synthase has at least 90% identity to a polypeptide sequence selected from the group consisting of SEQ ID NO: 3 and 5 
     
     
         2 . (canceled) 
     
     
         3 . The polynucleotide of  claim 1 , wherein the transit peptide is a chloroplast transit peptide. 
     
     
         4 . The polynucleotide of  claim 3 , wherein the chloroplast transit peptide is selected from the group consisting of an EPSPS chloroplast transit peptide, a small subunit ribulose-1,5,-bisphosphate carboxylase chloroplast transit peptide, a ferredoxin chloroplast transit peptide, a ferredoxin oxidoreductase chloroplast transit peptide, a light-harvesting complex protein I and protein II chloroplast transit peptide, and a thioredoxin F chloroplast transit peptide. 
     
     
         5 . The polynucleotide of  claim 1 , wherein the sequence encoding the plant methylketone synthase exhibits at least about 80% percent sequence identity to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 1, 2 and 4. 
     
     
         6 . A construct comprising the polynucleotide of  claim 1  operably linked to a promoter functional in plants. 
     
     
         7 . A plant cell comprising the polynucleotide of  claim 1 . 
     
     
         8 . The plant cell of  claim 7 , wherein the transit peptide is a chloroplast transit peptide. 
     
     
         9 . The plant cell of  claim 7 , wherein said plant cell is from a seed, root, leaf, shoot, flower, pollen, or ovule. 
     
     
         10 . The plant cell of  claim 7 , wherein said cell produces a methylketone. 
     
     
         11 . The plant cell of  claim 10 , wherein said methylketone is 2-undecanon; 2-tridecanone, or 2-pentadecanone. 
     
     
         12 . The plant cell of  claim 7 , wherein said cell is a crop plant cell. 
     
     
         13 . The plant cell of  claim 7 , wherein said cell is from a plant selected from the group selected from cotton, soybean, canola, corn, wheat, rice, sunflower, sorghum, sugarcane, potato, tomato, and a tree. 
     
     
         14 . A plant or a part thereof comprising the polynucleotide of  claim 1 . 
     
     
         15 . The plant or part thereof of  claim 14 , wherein the part thereof is selected from the group consisting of a seed, pollen, a root, a leaf, a shoot, a flower and an ovule. 
     
     
         16 . A processed product comprising a plant tissue comprising the polynucleotide of  claim 1 . 
     
     
         17 . The processed product of  claim 16 , selected from the group consisting of meal, flour, oil, hay, starch, juice, protein extract, and fiber. 
     
     
         18 . A method for controlling a pathogen or pest in a plant comprising expressing in the plant the construct of  claim 6 . 
     
     
         19 . The method for controlling a pathogen or pest in a plant of  claim 18 , wherein the polynucleotide sequence comprises a sequence that encodes a second heterologous transit peptide operably linked to a sequence that encodes an acyl carrier protein. 
     
     
         20 . The method of  claim 19 , wherein the pathogen or pest is a nematode. 
     
     
         21 . The method of  claim 20 , wherein the nematode is selected from the group consisting of  Heterodera  species,  Globodera  species,  Meloidogyne  species,  Rotylenchulus  species,  Hoplolaimus  species,  Belonolaimus  species,  Pratylenchus  species,  Longidorus  species,  Paratrichodorus  species,  Ditylenchus  species,  Xiphinema  species,  Dolichodorus  species,  Helicotylenchus  species,  Radopholus  species,  Hirschmanniella  species,  Tylenchorhynchus  species, and  Trichodorus  species. 
     
     
         22 . The method of  claim 19 , wherein the pathogen or pest is an insect pest. 
     
     
         23 . The method of  claim 22 , wherein the insect pest is selected from the group consisting of  Diabrotica, Diaprepes, Pachnaeus, Asynonychus, Lycoriella, Sciara, Stenophlus , and  Bradysia.    
     
     
         24 . A method of producing seed, comprising crossing the plant of  claim 14  with itself or a second plant. 
     
     
         25 . The method of producing seed of  claim 24 , wherein the polynucleotide sequence comprises a sequence that encodes a second heterologous transit peptide operably linked to a sequence that encodes an acyl carrier protein. 
     
     
         26 . The polynucleotide of  claim 1 , further comprising a sequence that encodes a second heterologous transit peptide operably linked to a sequence that encodes an acyl carrier protein. 
     
     
         27 . The polynucleotide of  claim 26 , wherein said sequence that encodes a second heterologous transit peptide operably linked to a sequence that encodes an acyl carrier protein encodes a polypeptide that comprises an amino acid sequence exhibiting at least about 85% identity to a polypeptide selected from the group consisting of SEQ ID NO: 41, 43, 45, 47, 49, 51, 53, 55, and 57. 
     
     
         28 . The polynucleotide of  claim 26 , wherein said second transit peptide is a chloroplast transit peptide. 
     
     
         29 . The polynucleotide of  claim 26 , wherein said second transit peptide is selected from the group consisting of an EPSPS chloroplast transit peptide, a small subunit ribulose-1,5,-bisphosphate carboxylase chloroplast transit peptide, a ferredoxin chloroplast transit peptide, a ferredoxin oxidoreductase chloroplast transit peptide, a light-harvesting complex protein I and protein II chloroplast transit peptide, and a thioredoxin F chloroplast transit peptide. 
     
     
         30 . The polynucleotide of  claim 26 , wherein said sequence that encodes a second heterologous transit peptide operably linked to a sequence that encodes an acyl carrier protein exhibits at least about 80% sequence identity to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 40, 42, 44, 46, 48, 50, 52, 54, and 56. 
     
     
         31 . The plant cell of  claim 7 , wherein the polynucleotide sequence comprises a sequence that encodes a second heterologous transit peptide operably linked to a sequence that encodes an acyl carrier protein. 
     
     
         32 . The plant or part thereof of  claim 14 , wherein the polynucleotide sequence comprises a sequence that encodes a second heterologous transit peptide operably linked to a sequence that encodes an acyl carrier protein. 
     
     
         33 . A processed product of a plant, plant part, seed or progeny, wherein the product comprises the plant cell of  claim 7 . 
     
     
         34 . The processed product of  claim 33 , wherein the processed product is selected from the group consisting of meal, flour, oil, hay, starch, juice, protein extract, and fiber. 
     
     
         35 . The polynucleotide of  claim 1 , wherein the polypeptide comprises an amino acid sequence with at least 95% identity to a polypeptide sequence selected from the group consisting of SEQ ID NO: 3 and 5.