Device and method for detection of analytes

Abstract

The present invention provides assays and devices for detection of substances in liquid samples. The assays and devices utilize passive diffusion between a porous material and a porous membrane containing a specific binding pair member to enable detection of the substance of interest.

1 . A device for detecting at least one substance of interest in a liquid sample, the device comprising:
 a receptacle comprising a porous material for receiving the liquid sample comprising or suspected of comprising  C. difficile  glutamate dehydrogenase, toxin A, and toxin B, wherein the porous material is capable of absorbing and transmitting at least a portion of the liquid sample; and   a porous membrane that comprises at least one specific binding pair member that is specific for  C. difficile  glutamate dehydrogenase, toxin A, and toxin B, wherein the at least one specific binding pair member includes a polyclonal antibody specific for toxin B.   
     
     
         2 . The device of  claim 1 , further comprising a container containing the receptacle. 
     
     
         3 . The device of  claim 1 , further comprising a holder for the porous membrane. 
     
     
         4 . The device of  claim 1 , further comprising a wash solution receiving pad. 
     
     
         5 . The device of  claim 1 , further comprising a liquid sample application pad. 
     
     
         6 . The device of  claim 1 , wherein the porous membrane that comprises at least one specific binding pair member includes a specific binding pair member including a monoclonal antibody specific for Toxin A. 
     
     
         7 . The device of  claim 1 , wherein the porous membrane that comprises at least one specific binding pair member includes a conjugate, wherein the conjugate includes the polyclonal antibody specific for Toxin B coupled to horseradish peroxidase, a monoclonal antibody specific for Toxin A coupled to horseradish peroxidase, and one or more antibodies specific for glutamate dehydrogenase coupled to horseradish peroxidase.