Hyaluronic acid formulation containing pyruvate

Abstract

Pyruvate may be used to stabilize hyaluronic acid compositions. For example, these compositions may have improved heat and/or storage stability.

1 . A composition comprising: a hyaluronic acid and pyruvate, wherein the hyaluronic acid is crosslinked, the composition suitable for use as a dermal filler, and the composition is a gel that is stable to heat sterilization. 
     
     
         2 . The composition of any one of the preceding claims, wherein the gel has improved thermal stability as compared to a substantially identical gel lacking pyruvate. 
     
     
         3 . The composition of any one of the preceding claims, wherein the gel has a Δ Tan δ 1 Hz of less than about 0.004 after heat sterilization. 
     
     
         4 . The composition of any one of the preceding claims, wherein the gel has a Δ Tan δ 1 Hz of less than about −0.03 after storage for 60 days at 45° C. 
     
     
         5 . A composition prepared by a process comprising: heat treating a gel comprising a crosslinked hyaluronic acid and a pyruvate. 
     
     
         6 . The composition of any one of the preceding claims, further comprising lidocaine. 
     
     
         7 . The composition of  claim 6 , wherein lidocaine is present at a concentration of about 0.05% w/w to about 1% w/w. 
     
     
         8 . The composition of any one of the preceding claims, wherein hyaluronic acid is present at a concentration of about 0.1% w/v. 
     
     
         9 . The composition of any one of the preceding claims, wherein pyruvate is present at a concentration of about 0.01% w/w to about 2% w/w. 
     
     
         10 . The composition of  claim 9 , wherein the pyruvate is present at a concentration of about 0.05% w/w to about 1% w/w. 
     
     
         11 . The composition of any one of the preceding claims, further comprising 3-aminopropyl-L-ascorbylphosphate. 
     
     
         12 . A dermal filler product comprising a composition of any one of the preceding claims. 
     
     
         13 . A method of improving the thermal stability of a dermal filler product, comprising: forming a gel comprising a combination of pyruvate and a crosslinked hyaluronic acid; wherein pyruvate is effective to improve the thermal stability of the gel. 
     
     
         14 . The method of claim  17 , wherein pyruvate has a concentration of about 0.1% w/w to about 1% w/w in the gel.