Preparation and/or Purification of Oligonucleotide Conjugates

Abstract

Methods, systems and/or kits for the preparation, purification and isolation of oligonucleotide conjugates, comprising conjugation of modified antibodies or proteins with at least one modified oligonucleotide at greater than 80% efficiency to form oligonucleotide conjugates and isolating the oligonucleotide conjugates from the conjugation solution by binding the conjugates to an immobilized binder, wherein the binder may be a metal ion or an antibody.

1 . A method for isolating antibody-oligonucleotide conjugates, comprising:
 i) conjugating a modified antibody with at least one modified oligonucleotide to form antibody-oligonucleotide conjugates, wherein greater than 80% of the modified antibodies are conjugated;   ii) adding the conjugation reaction mixture to a column having a stationary phase comprising a binder that has been immobilized to the stationary phase;   iii) binding the antibody-oligonucleotide conjugates selectively to the immobilized binder;   iv) eluting reaction components away from the bound antibody-oligonucleotide conjugates; and   v) isolating the antibody-oligonucleotide conjugates by releasing the bound antibody-oligonucleotide conjugates with a displacing agent selective for the binder.   
     
     
         2 - 57 . (canceled)