Process for Analyzing Sample by Capillary Electrophoresis Method

Abstract

A process for analyzing a sample by a capillary electrophoresis method is provided that allows for high analytic precision and reduction in apparatus size, and can be readily carried out by electrophoresing a complex of a sample and an anionic group-containing compound in the capillary channel, wherein the capillary channel includes an A layer that is coated on an inner wall thereof and a B layer that is coated on the A layer, where the A and B layers are as described.

1 - 18 . (canceled) 
     
     
         19 . A process for analyzing hemoglobin in a sample by a capillary electrophoresis method comprising:
 providing a sample comprising hemoglobin,   preparing a capillary channel for capillary electrophoresis, and   performing electrophoretic separation of a chemical complex comprising the hemoglobin and a first anionic compound that is present in a running buffer solution at a pH in a range of 4.5 to 6 in the capillary channel,   wherein the capillary channel comprises an inner wall coated with an A layer and a B layer that coats the A layer, wherein the A layer is covalently bonded to the inner wall and comprises at least one of a nonpolar polymer and a cationic compound, and the B layer comprises a second anionic compound,   wherein the first anionic compound may be the same or different from the second anionic compound.   
     
     
         20 . The process of  claim 19 , wherein the nonpolar polymer of the A layer is a silicone polymer. 
     
     
         21 . The process of  claim 20 , wherein the silicone polymer is selected from the group consisting of a polysiloxane and a polysilazane. 
     
     
         22 . The process of  claim 20 , wherein the silicone polymer is selected from the group consisting of a polydialkylsiloxane, a polydialkylsilazane, a polyarylsiloxane, a polyarylsilazane, a polyalkylarylsiloxane, a polydiarylsiloxane, a cyclic siloxane and a cyclic silazane. 
     
     
         23 . The process of  claim 19 , wherein the cationic compound of the A layer comprises a silylation agent containing at least one selected from an amino group and an ammonium group. 
     
     
         24 . The process of  claim 23 , wherein the silylation agent is selected from the group consisting of N-(2-diaminoethyl)-3-propyltrimethoxysilane, aminophenoxydimethylvinylsilane, 3-aminopropyldiisopropylethoxysilane, 3-aminopropylmethylbis(trimethylsiloxy)silane, 3-aminopropylpentamethyldisiloxane, 3-aminopropylsilanetriol, bis(p-aminophenoxy)dimethylsilane, 1,3-bis(3-aminopropyl)tetramethyldisiloxane, bis(dimethylamino)dimethylsilane, bis(dimethylamino)vinylmethylsilane, bis(2-hydroxyethyl)-3-aminopropyltriethoxysilane, 3-cyanopropyl(diisopropyl)dimethylaminosilane, (aminoethylaminomethyl)phenethyltrimethoxysilane, N-methylaminopropyltriethoxysilane, tetrakis(diethylamino)silane, tris(dimethylamino)chlorosilane and tris(dimethylamino)silane. 
     
     
         25 . The process of  claim 19 , wherein at least one of the first anionic compound and the second anionic compound is an anionic polysaccharide. 
     
     
         26 . The process of  claim 25 , wherein the anionic polysaccharide is at least one selected from the group consisting of a sulfated polysaccharide, a carboxylated polysaccharide, a sulfonated polysaccharide and a phosphorylated polysaccharide. 
     
     
         27 . The process of  claim 26 , wherein the sulfated polysaccharide is chondroitin sulfate. 
     
     
         28 . The process of  claim 19 , wherein the first anionic compound is chondroitin sulfate. 
     
     
         29 . The process of  claim 19 , wherein the second anionic compound is chondroitin sulfate. 
     
     
         30 . The process of  claim 19 , wherein the A layer comprises a nonpolar polymer. 
     
     
         31 . The process of  claim 19 , wherein the A layer comprises a cationic compound. 
     
     
         32 . The process of  claim 19 , wherein the A layer consists of a cationic compound. 
     
     
         33 . The process of  claim 19 , wherein the hemoglobin is a glycated hemoglobin. 
     
     
         34 . The process of  claim 19 , wherein the hemoglobin is HbA1c.