Process for the preparation of herbal extract of cassia tora leaves for treating anxiety disorders

Abstract

The present invention relates to novel use of the extracts of Cassia tora plant species. The present invention particularly relates to novel use of the extracts of Cassia tora leaves, which show antianxiety activity. The present invention also relates to a process for the preparation of novel herbal extract of Cassia tora species with antianxiety activity and also to the use thereof for the treatment of anxiety.

1 .- 7 . (canceled) 
     
     
         8 . A method of treating anxiety disorders comprising the administration of an effective amount of an herbal extract of  Cassia tora  leaves, the preparation comprising the steps of:
 i. collecting  Cassia tora  plant material;   ii. drying the plant material at ambient temperature in the range of 25 to 37° C. while maintaining the moisture in the range of 0.5-1.5 percent;   iii. pulverizing the plant material dried in step (ii) and selecting the material in the size range of 16-20 mesh sieve;   iv. soaking the pulverized plant material obtained in step (iii) above in 95:05 (v/v) alcohol-water mixture in static condition at ambient temperature followed by draining & collecting the extract solution;   v. repeating the soaking step described in step (iv) above five times with fresh solvent mixture at an interval of 24 hours;   vi. concentrating the extract solution obtained in step (v) by known methods; and   vii. freeze drying the concentrated extract obtained in step (vi) at a temperature in the range of −50 to −60° C. for a period in the range of 8 to 16 hours.   
     
     
         9 . The method of  claim 8 , wherein a plant part used is leaves of  Cassia tora.    
     
     
         10 . The method of  claim 8 , wherein the extract obtained by said process is active on benzodiazepine receptors. 
     
     
         11 . The method of  claim 8 , wherein the extract obtained by said process exhibits inhibition for binding to benzodiazepine receptors even at concentration as low as 1.56 μg. 
     
     
         12 . The method of  claim 8 , wherein the extract obtained by said process is administered in a form selected from tablets, lozenges, capsules, powder, solution, intravenously and orally.