Recombinase polymerase amplification reagents and kits

Abstract

This disclosure describes kits, reagents and methods for Recombinase Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed kits, reagents and methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods.

1 - 39 . (canceled) 
     
     
         40 . A frieze dried pellet for a recombinase polymerase amplification process of DNA amplification of a target nucleic acid molecule, comprising: trehalose, and wherein said pellet does not contain polyethylene glycol. 
     
     
         41 . The frieze dried pellet of  claim 40 , wherein said pellet comprises 2.5%-7.5% weight/lyophilization mixture volume of trehalose.