Engineered yeast for cellulosic ethanol production

Abstract

The disclosure provides designer cellulosomes for efficient hydrolysis of cellulosic material and more particularly for the generating of ethanol.

What is claimed is: 
     
         1 . A recombinant yeast cell comprising:
 a heterologous polynucleotide that encodes a secreted polypeptide comprising a dockerin domain, a scaffoldin polypeptide, one or more cellulose binding domains (CBD) and a plurality of divergent-heterologous cohesion domains.   
     
     
         2 . The recombinant yeast cell of  claim 1 , wherein the polypeptide comprises from N- to C-terminus: the dockerin polypeptide, the plurality of divergent-heterologous cohesion domains and the CBD. 
     
     
         3 . The recombinant yeast cell of  claim 1 , wherein the dockerin domain is from  B. cellulosolvens  or  C. acetobutylicum.    
     
     
         4 . A recombinant yeast cell comprising:
 a heterologous polynucleotide encoding a polypeptide comprising a surface anchor antigen (AGA) domain, a scaffoldin domain and at least one cohesion domain.   
     
     
         5 . The recombinant yeast cell of  claim 4 , wherein the AGA domain is AGA1 or AGA2.