Design, synthesis and use of synthetic nucleotides comprising charge mass tags

Abstract

Embodiments of the present disclosure relate generally to reporter compositions which are synthetic nucleotides that comprise nucleotides with a high charge mass moiety attached thereto via a linker molecule. The linker molecules can vary in length in part to enable the high charge mass moiety to extend out from a DNA polymerase complex so that polymerization may not be influenced.

1 . A reporter composition, comprising:
 a nucleotide or its derivative;   a linker molecule, wherein the linker molecule is attached to the nucleotide or its derivative and a high charge mass moiety; and   a high charge mass moiety, wherein the high charge mass moiety comprises a charge mass that is sufficient to change a property of a sensitive detection nanostructure operably coupled to the reporter composition.   
     
     
         2 . The reporter composition of  claim 1 , wherein the nucleotide or its derivative is selected from the group consisting of a deoxyribonucleotide, a ribonucleotide, a peptide nucleotide, a morpholino, a locked nucleotide, a glycol nucleotide, a threose nucleotide, any synthetic nucleotides, any isoforms thereof, and any derivatives thereof. 
     
     
         3 . The reporter composition of  claim 1 , wherein the linker molecule comprises a molecule of the following general formula:
   H 2 N-L-NH 2      
       wherein L comprises a linear or branched chain comprising an alkyl group, an oxy alkyl group, or the combination thereof. 
     
     
         4 . The reporter composition of  claim 3 , wherein L comprises a linear chain comprising an alkyl group, an oxy alkyl group, or the combination thereof. 
     
     
         5 . The reporter composition of  claim 4 , wherein a number of the alkyl group, the oxy alkyl group, or the combination thereof in the linear chain is 1 to 100. 
     
     
         6 . The reporter composition of  claim 5 , wherein the number of the alkyl group, the oxy alkyl group, or the combination thereof in the linear chain is 1 to 75. 
     
     
         7 . The reporter composition of  claim 5 , wherein the number of the alkyl group, the oxy alkyl group, or the combination thereof in the linear chain is 1 to 50. 
     
     
         8 . The reporter composition of  claim 5 , wherein the number of the alkyl group, the oxy alkyl group, or the combination thereof in the linear chain is 1 to 25. 
     
     
         9 . The reporter composition of  claim 5 , a number of the alkyl group, the oxy alkyl group, or the combination thereof in the linear chain is 1 to 1000. 
     
     
         10 . The reporter composition of  claim 5 , a number of the alkyl group, the oxy alkyl group, or the combination thereof in the linear chain is more than 1000. 
     
     
         11 . The reporter composition of  claim 1 , wherein the linker molecule is configured not to affect nucleotide polymerization by a polymerase. 
     
     
         12 . The reporter composition of  claim 1 , wherein a net charge mass of the high charge mass moiety is positive or negative. 
     
     
         13 . The reporter composition of  claim 1 , wherein a net charge mass of the high charge mass moiety is variable depending on pH. 
     
     
         14 . The reporter composition of  claim 1 , wherein the high charge mass moiety comprises an aromatic and/or aliphatic skeleton, wherein said skeleton comprises one or more of a tertiary amino group, an alcohol hydroxyl group, a phenolic hydroxy group, and any combinations thereof. 
     
     
         15 . The reporter composition of  claim 1 , wherein the high charge mass moiety comprises one or more of the following groups, and derivatives and combinations thereof: 
       
         
           
           
               
               
           
         
       
     
     
         16 . The reporter composition of  claim 15 , wherein a number of the groups, any derivatives thereof, and any combinations thereof in the high charge mass moiety is 1 to 10. 
     
     
         17 . The reporter composition of  claim 15 , wherein a number of the groups, any derivatives thereof, and any combinations thereof in the high charge mass moiety is 11 to 50. 
     
     
         18 . The reporter composition of  claim 15 , wherein a number of the groups, any derivatives thereof, and any combinations thereof in the high charge mass moiety is 51 to 100. 
     
     
         19 . The reporter composition of  claim 15 , wherein a number of the groups, any derivatives thereof, and any combinations thereof in the high charge mass moiety is more than 100. 
     
     
         20 . The reporter composition of  claim 1 , wherein the high charge mass moiety is configured not to affect nucleotide polymerization by a polymerase. 
     
     
         21 - 28 . (canceled)