Renovation and Repopulation of Decellularized Tissues and Cadaveric Organs by Stem Cells

Abstract

A method of manufacturing a tissue matrix for implantation into a patient is disclosed. The method sets forth collecting embryonic stem cells from a placenta which has been treated to remove residual cord blood and seeding the collected stem cells onto or into a tissue matrix. The seeded tissue matrix is then implanted on or into a patient. The seeded tissue matrix made by the method of the present invention is also disclosed.

1 .- 23 . (canceled) 
     
     
         24 . A tissue comprising a decellularized organ and a plurality of non-placental cells. 
     
     
         25 . The tissue of  claim 24 , wherein said decellularized organ is a placenta. 
     
     
         26 . The tissue of  claim 24 , wherein said decellularized organ is a heart. 
     
     
         27 . The tissue of  claim 24 , wherein the non-placental cells are stem cells or progenitor cells. 
     
     
         28 . The tissue of  claim 27 , wherein the progenitor cells are progenitor cells for neurons, muscle, blood, bone, bone marrow, skin, heart, connective tissue, lung, kidney, liver, or pancreas. 
     
     
         29 . The tissue of  claim 28 , wherein said progenitor cells for pancreas are progenitor cells for pancreatic islet cells. 
     
     
         30 . The tissue of  claim 27 , wherein the stem cells are mesenchymal stem cells. 
     
     
         31 . The tissue of  claim 30 , wherein said mesenchymal stem cells are from bone marrow. 
     
     
         32 . The tissue of  claim 30 , wherein said mesenchymal stem cells are from embryonic yolk sac, umbilical cord, fetal skin, adolescent skin, or blood. 
     
     
         33 . The tissue of  claim 24 , wherein the non-placental cells are on or more of hematopoietic cells, neuron cells, fibroblast cells, strand cells, or hepatic cells. 
     
     
         34 . The tissue of  claim 24 , wherein the non-placental cells are stromal cells, endothelial cells, hepatocytes, or keratinocytes. 
     
     
         35 . The tissue of  claim 24 , wherein the non-placental cells are cells that have been differentiated from placental stem cells. 
     
     
         36 . The tissue of  claim 35 , wherein the cells that have been differentiated from placental stem cells are adipogenic cells, chondrogenic cells, osteogenic cells, myogenic cells, vasogenic cells, neurogenic cells, or hepatogenic cells.