Isolation of adult multipotential cells by tissue non-specific alkaline phosphatase

Abstract

The present invention relates to the use of tissue non-specific alkaline phosphatase (TNAP) as a marker for identifying and/or isolating adult multipotential cells. The present invention also relates to cell populations enriched by methods of the present invention and therapeutic uses of these cells.

1 . Use of TNAP as a marker for the identification and/or enrichment of adult multipotential cells. 
     
     
         2 - 17 . (canceled) 
     
     
         18 . An enriched population of adult multipotential cells obtained by a method according to  claim 1 . 
     
     
         19 . An enriched population of TNAP+ adult multipotential cells. 
     
     
         20 . An expanded cell population obtained by culturing an enriched population of adult multipotential cells according to  claim 18 . 
     
     
         21 - 24 . (canceled) 
     
     
         25 . A composition comprising a population of enriched adult multipotential cells according to  claim 18 . 
     
     
         26 - 28 . (canceled) 
     
     
         29 . A method for generating or repairing tissue in a subject, the method comprising administering to the subject an enriched or expanded cell population according to  claim 18 . 
     
     
         30 . A method for generating or repairing tissue in a subject, the method comprising administering to the subject a composition according to  claim 25 . 
     
     
         31 . A method according to  claim 29  wherein the tissue is bone, cardiac or cartilage tissue. 
     
     
         32 - 40 . (canceled) 
     
     
         41 . A STRO-3 hybridoma cell line deposited with ATCC on 19 Dec. 2005 under the provisions of the Budapest Treaty under deposit accession number PTA-7282. 
     
     
         42 . A STRO-3 antibody produced by the hybridoma cell line deposited with ATCC on 19 Dec. 2005 under the provisions of the Budapest Treaty under deposit accession number PTA-7282. 
     
     
         43 . An isolated antibody which binds to the same epitope on multipotential cells as the STRO-3 antibody produced by the hybridoma cell line deposited with ATCC on 19 Dec. 2005 under the provisions of the Budapest Treaty under deposit accession number PTA-7282. 
     
     
         44 . A composition comprising an antibody according to  claim 42  or  claim 43 . 
     
     
         45 . (canceled) 
     
     
         46 . A method for generating or repairing tissue in a subject, the method comprising administering to the subject an enriched or expanded cell population according to  claim 19 . 
     
     
         47 . A method for generating or repairing tissue in a subject, the method comprising administering to the subject an enriched or expanded cell population according to  claim 20 .