Method for separating estrogen from placenta

Abstract

The present invention provides a method of separating estrogen from placenta, which uses supercritical fluid technique to load the placenta powder of human body or sheep, pig, deer and other animals into an extraction tank. Under the operating conditions of preset pressure and temperature, supercritical solvent is added into the extraction tank to extract estrogen from placenta, so as to acquire de-estrogen placenta powder and placenta extract liquor. Under the same condition, the de-estrogen placenta extract liquor and supercritical solvent are added by a preset volume flow ratio into an adsorption tank. The estrogen in the placenta extract liquor is adsorbed by the adsorption tank to obtain de-estrogen placenta extract. It is then eluted with ethanol solution by gradient proportion to obtain purified natural estrogen.

1 . A method of separating estrogen from placenta using a supercritical fluid, comprising the following steps:
 extraction: under the operating conditions of preset temperature and pressure, load placenta powder in an extraction tank, add supercritical solvent to extract estrogen from placenta powder, and produce placenta extract liquor, wherein said placenta powder is a dry placenta powder of human body or sheep, pig, deer or other animals, and wherein said supercritical solvent is supercritical carbon dioxide/ethanol, and in the extraction step, it is required to load 1 Kg placenta powder in an extraction tank under the operating condition of 20-30 MPa and 40° C., add supercritical solvent into an adsorption tank by a volume flow ratio of 10˜20:1, extract estrogen after for 2-3 h reaction time;   adsorption: under the same operating condition, add the extract liquor of de-estrogen placenta and supercritical solvent into an adsorption tank, allow the estrogen in the placenta extract liquor to be adsorbed by the adsorption tank, obtain the de-estrogen placenta extract, wherein said adsorbent may be silica gel, sephadex or resin and other adsorbent that can adsorb estrogen; and   gradient elution: elute the estrogen in the adsorption tank with gradient proportion ethanol solution for desorption of the estrogen, obtain purified natural estrogen, wherein said ethanol solution is eluted by gradient proportion from 50% to 80%.   
     
     
         2 . (canceled) 
     
     
         3 . (canceled) 
     
     
         4 . The method defined in  claim 3 , wherein, in the extraction step, the optimal operating condition is: pressure of 24˜26 MPa, temperature of 40° C., and the volume flow ratio of supercritical solvent of 15:1, the rejection can reach 90% 
     
     
         5 . The method defined in  claim 3 , wherein said extraction tank is a stainless steel tank with an internal diameter of 60 mm, external diameter of 130 mm and height of 130 mm, filled with stainless steel monomer pieces. 
     
     
         6 . The method defined in  claim 4 , wherein said extraction tank is a stainless steel tank with an internal diameter of 60 mm, external diameter of 130 mm and height of 130 mm, filled with stainless steel monomer pieces. 
     
     
         7 . The method defined in  claim 1 , wherein, in the adsorption step, said adsorption tank is a stainless steel tank with an internal diameter of 36 mm, external diameter of 48 mm and height of 597 mm, filled with adsorbent that can adsorb estrogen. 
     
     
         8 . (canceled) 
     
     
         9 . (canceled) 
     
     
         10 . (canceled)