Technology for preparation of macromolecular microspheres

Abstract

Microspheres are produced by contacting an aqueous solution of a protein or other macromolecule with an organic solvent and a counterion, and chilling the solution. The microspheres are useful for preparing pharmaceuticals of defined dimensions.

1 . A method of making a protein-based composition, comprising:
 a) adding a counterion to a solution containing the protein in an aqueous solvent;   b) adding an organic solvent to the solution; and   c) gradually cooling the solution to a temperature below about 25° C., whereby a composition containing microparticles comprising the protein is formed, wherein steps a), b) and c) are performed simultaneously, sequentially, intermittently, or in any order.   
     
     
         2 .- 141 . (canceled)