Method for testing microfluids by a multichannel system with a multichannel disc

Abstract

The present invention discloses a system for testing microfluid which is made with a disposable disc. The high sensitivity, high sensing accuracy, and quick response microfluidic disc is demonstrated in the present invention. It is note that easy to test microfluid without traditional detecting method, and then reduce energy and simplify procedure. Furthermore, to additive the microfluidic disc is useful to enhance blood typing, and hence raising the sensitivity by the video recognition of blood agglutination.

What is claimed is: 
     
         1 . A method for testing microfluids by a multichannel system, comprising:
 performing a dosing procedure to respectively conduct a plurality of specimens and a plurality of microfluidic reagents to a plurality of feeding chambers of a disc;   controlling the speed of a spinning device to create different centrifugal powers to perform a centrifugal procedure on the disc and through this to control the microfluids discharging speeds in different feeding chambers, wherein a plurality of microfluids respectively go through a plurality of microfluidic channels in centrifugal state to a reaction chamber of the disc to proceed mixing to form a reactant in a testing zone in the reaction chamber; and   performing a testing procedure by an image collecting device to observe and measure the reactant's coagulation state.   
     
     
         2 . The method for testing microfluids by a multichannel system according to  claim 1 , wherein the centrifugal procedure comprises:
 performing a first centrifugal step to conduct a first, a second, and a third microfluids inside the feeding chambers to the reaction chamber;   performing a first mixing and blending procedure in the reaction chamber through reciprocating the disc by the spinning device to mix and blend the first, the second and the third microfluids to form a first reactant;   performing a second centrifugal step to conduct a fourth microfluid from one of the feeding chambers to the reaction chamber;   performing a second mixing and blending procedure in the reaction chamber through reciprocating the disc by the spinning device to mix and blend the fourth microfluid with the first reactant to form a second reactant and increasing coagulation reaction;   performing a third centrifugal step to separate the coagulation in the second reactant, and leaving most of the specimens and coagulations of other reagents in the testing zone to be mixed and formed a third reactant;   performing a fourth centrifugal step to conduct a fifth microfluid from one of the feeding chambers to the reaction chamber to mix with the third reactant to form a fourth reactant; and   performing a third mixing and blending procedure to coagulate the fourth reactant.   
     
     
         3 . The method for testing microfluids by a multichannel system according to  claim 2 , wherein the first microfluid is RBC, the second microfluid is antibody/plasma, the third microfluid is LIM, the fourth microfluid is polybrene/polythyleneimine, and the fifth microfluid is resuspending solution. 
     
     
         4 . The method for testing microfluids by a multichannel system according to  claim 2 , wherein the centrifugal speed of the first centrifugal step is 600-800 rpm and the operating time is 1-10 sec; the centrifugal speed of the second centrifugal step is 600-1000 rpm and the operating time is 1-10 sec; the centrifugal speed of the third centrifugal step is 600-1500 rpm and the operating time is 1-10 sec; and the centrifugal speed of the fourth centrifugal step is 600-2000 rpm and the operating time is 1-10 sec. 
     
     
         5 . The method for testing microfluids by a multichannel system according to  claim 2 , wherein the revolving angle range of reciprocating for the first, the second and the third mixing and blending procedure is from 10 to 360 degrees, and the reciprocating time is 1-10 times. 
     
     
         6 . The method for testing microfluids by a multichannel system according to  claim 1 , wherein the testing procedure produces an image signal by the image collecting device based on the coagulation state of the reactants to perform a matching and analyzing procedure, wherein the matching and analyzing procedure is done through a matching and analyzing device by matching and analyzing the image signal with the data in a default database to confirm the coagulation state of the fourth reactant. 
     
     
         7 . The method for testing microfluids by a multichannel system according to  claim 1 , wherein the feeding chamber connects with an exterior atmosphere under centrifugal state to make the feeding chamber form a vent chamber to allow the microfluids to flow under centrifugal state. 
     
     
         8 . The method for testing microfluids by a multichannel system according to  claim 1 , wherein the reaction chamber comprises at least one reaction testing zone, the reaction testing zone stores microfluidic reactants to facilitate the collection and analysis of the images of reactant coagulations. 
     
     
         9 . The method for testing microfluids by a multichannel system according to  claim 1 , further comprising at least one microfluidic channel to connect the feeding chamber and the reaction chamber and to transport the microfluids under centrifugal state, wherein a specific joint angle at the connection between the microfluidic channel and the feeding chamber controls discharging rate of the microfluids through a centrifugal rotational speed and prevents reverse flow of the microfluids. 
     
     
         10 . The method for testing microfluids by a multichannel system according to  claim 9 , wherein the microfluidic channel has a specific bending angle which can control discharging rate of the microfluids through the centrifugal rotational speed and prevent reverse flow of the microfluids. 
     
     
         11 . The method for testing microfluids by a multichannel system according to  claim 9 , wherein the reaction chamber further comprises at least one waste chamber to receive waste fluid from the reaction chamber. 
     
     
         12 . The method for testing microfluids by a multichannel system according to  claim 9 , wherein a capillary valve at the connection between the microfluidic channel and the feeding chamber controls discharging rate of the microfluids through the centrifugal rotational speed and prevents reverse flow of the microfluids. 
     
     
         13 . The method for testing microfluids by a multichannel system according to  claim 12 , wherein the capillary valve forms an interlocked combination of at least one convex channel and at least one cavernous channel on the microfluidic channel to form a serrated appearance, wherein the diameter of the convex channel is bigger than that of the cavernous channel, and the diameter of the convex channel is also bigger than that of the microfluidic channel. 
     
     
         14 . A method for testing microfluids by a multichannel disc, comprising:
 controlling the speed of a spinning device to create different centrifugal powers to perform a centrifugal procedure on the disc and through this to control the microfluids discharging speeds in different feeding chambers, wherein a plurality of microfluids respectively go through a plurality of microfluidic channels in centrifugal state to a reaction chamber of the disc to proceed mixing to form a reactant in a testing zone in the reaction chamber, wherein the centrifugal procedure comprises:   performing a first centrifugal step to conduct a first, a second, and a third microfluids inside the feeding chambers to the reaction chamber;   performing a first mixing and blending procedure in the reaction chamber through reciprocating the disc by the spinning device to mix and blend the first, the second and the third microfluids to form a first reactant;   performing a second centrifugal step to conduct a fourth microfluid from one of the feeding chambers to the reaction chamber;   performing a second mixing and blending procedure in the reaction chamber through reciprocating the disc by the spinning device to mix and blend the fourth microfluid with the first reactant to form a second reactant and increasing coagulation reaction;   performing a third centrifugal step to separate the coagulation in the second reactant, and leaving most of the specimens and coagulations of other reagents in the testing zone to be mixed and formed a third reactant;   performing a fourth centrifugal step to conduct a fifth microfluid from one of the feeding chambers to the reaction chamber to mix with the third reactant to form a fourth reactant; and   performing a third mixing and blending procedure to coagulate the fourth reactant.   
     
     
         15 . The method for testing microfluids by a multichannel disc according to  claim 14 , wherein the method for testing microfluids by multichannel disc comprising:
 performing a dosing procedure to respectively conduct a plurality of specimens and a plurality of microfluidic reagents to a plurality of feeding chambers of a disc; and   performing a testing procedure by an image collecting device to observe and measure the reactant's coagulation state.   
     
     
         16 . The method for testing microfluids by multichannel disc according to  claim 14 , wherein the first microfluid is RBC, the second microfluid is antibody/plasma, the third microfluid is LIM, the fourth microfluid is polybrene/polythyleneimine, and the fifth microfluid is resuspending solution. 
     
     
         17 . The method for testing microfluids by a multichannel disc according to  claim 14 , wherein the centrifugal speed of the first centrifugal step is 600-800 rpm and the operating time is 1-10 sec; the centrifugal speed of the second centrifugal step is 600-1000 rpm and the operating time is 1-10 sec; the centrifugal speed of the third centrifugal step is 600-1500 rpm and the operating time is 1-10 sec; and the centrifugal speed of the fourth centrifugal step is 600-2000 rpm and the operating time is 1-10 sec, wherein the revolving angle range of reciprocating for the first, the second and the third mixing and blending procedure is from 10 to 360 degrees, and the reciprocating time is 1-10 times, wherein the testing procedure produces an image signal by the image collecting device based on the coagulation state of the reactants to perform a matching and analyzing procedure, wherein the matching and analyzing procedure is done through a matching and analyzing device by matching and analyzing the image signal with the data in a default database to confirm the coagulation state of the fourth reactant. 
     
     
         18 . The method for testing microfluids by a multichannel disc according to  claim 14 , wherein the feeding chamber connects with an exterior atmosphere under centrifugal state to make the feeding chamber form a vent chamber to allow the microfluids to flow under centrifugal state. 
     
     
         19 . The method for testing microfluids by a multichannel disc according to  claim 14 , wherein the reaction chamber comprises at least one reaction testing zone, the reaction testing zone stores microfluidic reactants to facilitate the collection and analysis of the images of reactant coagulations. 
     
     
         20 . The method for testing microfluids by a multichannel disc according to  claim 14 , further comprising at least one microfluidic channel to connect the feeding chamber and the reaction chamber and to transport the microfluids under centrifugal state, wherein a specific joint angle at the connection between the microfluidic channel and the feeding chamber controls discharging rate of the microfluids through a centrifugal rotational speed and prevents reverse flow of the microfluids, wherein the microfluidic channel has a specific bending angle which can control discharging rate of the microfluids through the centrifugal rotational speed and prevent reverse flow of the microfluids, wherein the reaction chamber further comprises at least one waste chamber to receive waste fluid from the reaction chamber, wherein a capillary valve at the connection between the microfluidic channel and the feeding chamber controls discharging rate of the microfluids through the centrifugal rotational speed and prevents reverse flow of the microfluids, wherein the capillary valve forms an interlocked combination of at least one convex channel and at least one cavernous channel on the microfluidic channel to form a serrated appearance, wherein the diameter of the convex channel is bigger than that of the cavernous channel, and the diameter of the convex channel is also bigger than that of the microfluidic channel.