Enterococcus faecium ef08 strain, a use, a feed additive and a feed thereof

Abstract

An Enterococcus faecium EF08 strain for manufacturing a feed additive is disclosed. The EF08 strain is deposited at Bioresource Collection and Research Center of Taiwan with deposit number BCRC 910525. Also, the EF08 strain is deposited at CGMCC-China General Microbiological Culture Collection with deposit number CGMCC 5549. Moreover, the EF08 strain has a 16S rDNA sequence as set forth as SEQ ID NO: 1.

What is claimed is: 
     
         1 . An  Enterococcus faecium  EF08 strain, deposited at Bioresource Collection and Research Center of Taiwan with deposit number BCRC 910525, deposited at CGMCC-China General Microbiological Culture Collection with deposit number CGMCC 5549, and with a 16S rDNA sequence as set forth as SEQ ID NO: 1. 
     
     
         2 . A use of the EF08 strain described in  claim 1 , wherein the EF08 strain is used to manufacture a feed additive for terrestrial economic animals. 
     
     
         3 . The use according to  claim 2 , wherein the EF08 strain is incorporated into a feed for the terrestrial economic animals at dosages of 10 4 -10 8  CFU/per gram of the feed. 
     
     
         4 . A use of the EF08 strain described in  claim 1 , wherein the EF08 strain is used to enhance secretion of IL-10, TNF-α, IL-12p40 and IFN-γ. 
     
     
         5 . A use of the EF08 strain described in  claim 1 , wherein the EF08 strain is used to suppress growth of  Escherichia coli  and  Salmonella typhimurium.    
     
     
         6 . A feed additive comprising the EF08 strain described in  claim 1 , wherein the feed additive comprises the EF08 strain at concentrations of 10 8 -10 12  CFU/per gram of the feed additive. 
     
     
         7 . The feed additive according to  claim 6 , wherein the feed additive further comprises a  Lactobacillus  strain selected from a group consisting of a  Lactobacillus plantarum  LP28 strain and a  Lactobacillus acidophilus  LASW strain, wherein the LP28 strain is deposited at CGMCC-China General Microbiological Culture Collection with deposit number CGMCC 3346, and wherein the LASW strain is deposited at CGMCC-China General Microbiological Culture Collection with deposit number CGMCC M204083. 
     
     
         8 . The feed additive according to  claim 7 , wherein the feed additive comprises the  Lactobacillus  strain at concentrations of 10 8 -10 12  CFU/per gram of the feed additive. 
     
     
         9 . The feed additive according to  claim 8 , wherein the feed additive comprises the LP28 strain at concentrations of 10 8 -10 12  CFU/per gram of the feed additive and the LASW strain at concentrations of 10 8 -10 11  CFU/per gram of the feed additive. 
     
     
         10 . A feed comprising the EF08 strain described in  claim 1 , wherein the EF08 strain is at dosages of 10 4 -10 8  CFU/per gram of the feed.