Beautyberry total glycosides extract and preparation method and use thereof

Abstract

Disclosed is a composition comprising an beautyberry total glycosides extract, and a method of preparation thereof, and the use of the composition thereof in preparing drugs for treating neurodegenerative diseases or skin diseases. The extract thereof is prepared from the leaves of Callicarpa cathayana H. T. Chang or Callicarpa formosana Rolfe, and contains 18% to 45% verbascoside and 15% to 40% Arenarioside.

1 .- 10 . (canceled) 
     
     
         11 . A beautyberry total glycosides extract, containing 18% to 45% verbascoside and 15% to 40% Arenarioside based on weight, wherein the extract is prepared from leaves of  Callicarpa cathayana  H. T. Chang or  Callicarpa formosana  Rolfe. 
     
     
         12 . The beautyberry total glycosides extract according to  claim 11 , containing 28% to 45% verbascoside based on weight. 
     
     
         13 . The beautyberry total glycosides extract according to  claim 11 , containing 24% to 40% Arenarioside based on weight. 
     
     
         14 . The beautyberry total glycosides extract according to  claim 11  produced by:
 (1) pulverizing leaves of  Callicarpa cathayana  H. T. Chang or  Callicarpa formosana  Rolfe, and extracting with a solvent 1 to 3 times, where the solvent is water, alcohol, or a mixture of water and alcohol; 
 (2) combining extract liquors from each extraction, concentrating under reduced pressure to remove the organic solvent in step (1); adding therein water in an amount of 0.5 to 2 times the volume, standing overnight, centrifuging or filtering to obtain supernatant; and 
 (3a) passing the supernatant through a chromatographic column packed with a resin filler, washing with water and/or a dilute alcohol aqueous solution to remove impurities, then eluting with an alcohol aqueous solution at a higher concentration, collecting eluent, concentrating under reduced pressure, and drying, so as to obtain the beautyberry total glycosides extract; or 
 (3b) extracting the supernatant with an organic solvent, concentrating the organic phase under reduced pressure, and drying, so as to obtain the beautyberry total glycosides extract. 
 
     
     
         15 . The beautyberry total glycosides extract according to  claim 14 , wherein in step (1), the extraction method is selected from a flash extraction method, a reflux extraction method, a microwave extraction method, an ultrasonic extraction method and a percolation extraction method. 
     
     
         16 . The beautyberry total glycosides extract according to  claim 14 , wherein in step (3a), the resin filler is selected from a macroporous adsorbent resin, a polyamide resin and an ion exchange resin. 
     
     
         17 . The beautyberry to al glycosides extract according to  claim 14 , wherein in step (3a), the dilute alcohol aqueous solution is a 0 to 15 vol % dilute alcohol aqueous solution, and the alcohol aqueous solution at a higher concentration is a 30 to 90 vol % alcohol aqueous solution. 
     
     
         18 . The beautyberry total glycosides extract according to  claim 14 , wherein in step (3b), the organic solvent is n-butanol or ethyl acetate. 
     
     
         19 . The beautyberry total glycosides extract according to  claim 14 , wherein in step (3a), the following operation is performed one or more times after the concentration of the eluent under reduced pressure: the concentrated solution is dissolved with water, allowed to pass through the chromatographic column, washed with water and/or a dilute alcohol aqueous solution to remove impurities, and then eluted with an alcohol aqueous solution at a higher concentration, the eluent is collected and concentrated under reduced pressure. 
     
     
         20 . A method of treating a skin disease or a neurodegenerative disease in a subject in need thereof, the method comprising administering to the subject a composition comprising a beautyberry total glycosides extract containing 18% to 45% verbascoside and 15% to 40% Arenarioside based on weight and at least one pharmaceutically acceptable additive. 
     
     
         21 . The method according to  claim 20 , wherein the skin disease is dermatitis or eczema. 
     
     
         22 . The method according to  claim 20 , wherein the neurodegenerative disease is dementia, Parkinson's disease, or nerve injury. 
     
     
         23 . The method according to  claim 20 , wherein the extract is prepared from leaves of  Callicarpa cathayana  H. T. Chang or  Callicarpa formosana  Rolfe. 
     
     
         24 . The method according to  claim 20 , wherein the extract comprises 28% to 45% verbascoside based on weight. 
     
     
         25 . The method according to  claim 20  wherein the extract comprises 24% to 40% Arenarioside based on weight. 
     
     
         26 . The method according to  claim 20 , wherein the pharmaceutically acceptable additive is a binder, a lubricant, a disintegrating agent, a flavoring agent, an antioxidant, an emulsifying agent, a thickening agent, and/or a preservative.