Cochleates made with soy phosphatidylserine

Abstract

Unpurified or low pure soy phosphatidylserine is used to make cochleates. The cochleates contain about 40-74% soy phosphatidylserine, a multivalent cation and a biological active. A preferred cochleate contains the antifungal agent amphotericin B.

1 - 35 . (canceled) 
     
     
         36 . A crystal cochleate, comprising:
 a soy-based phospholipid comprising 50% by weight soy phosphatidylserine,   calcium, and   amphotericin B.   
     
     
         37 . A geode cochleate, comprising:
 a soy-based phospholipid that comprises 50% by weight soy phosphatidylserine,   calcium,   an oil, and   amphotericin B   
     
     
         38 . The crystal cochleate of claim  6 , wherein the ratio of the soy-based phospholipid to amphotericin B is about 5:1. 
     
     
         39 . The geode cochleate of  claim 37 , wherein the ratio of the soy-based phospholipid to amphotericin B is about 5:1. 
     
     
         40 . A method of making the crystal cochleate of  claim 36 , wherein the method comprises:
 (a) dissolving amphotericin B in a sodium hydroxide solution to form a basic amphotericin B solution;   (b) mixing the basic amphotericin B solution and the soy-based phospholipid comprising 50% by weight soy phosphatidylserine to form liposomes in an aqueous solution, wherein the liposomes contain amphotericin B; and   (c) adding calcium to the liposomes to form the crystal cochleate.   
     
     
         41 . The method of  claim 40 , wherein the ratio of the soy-based phospholipid to amphotericin B is about 5:1. 
     
     
         42 . The method of  claim 40 , wherein the aqueous solution is a phosphate buffered solution. 
     
     
         43 . The method of  claim 40 , further comprising adding an antioxidant to the liposomes before adding the calcium. 
     
     
         44 . The method of  claim 43 , further comprising lyophilizing the crystal cochleate. 
     
     
         45 . A method of making the geode cochleate of  claim 37 , wherein the method comprises:
 (a) dissolving amphotericin B in a sodium hydroxide solution to form a basic amphotericin B solution;   (b) mixing the basic amphotericin B solution with the oil and the soy-based phospholipid comprising 50% by weight soy phosphatidylserine to form geode liposomes in an aqueous solution, wherein the geode liposomes contain amphotericin B; and   (c) adding calcium to the geode liposomes to form the geode cochleate.   
     
     
         46 . The method of  claim 45 , wherein the ratio of the soy-based phospholipid to amphotericin B is about 5:1. 
     
     
         47 . The method of  claim 45 , further comprising adding an antioxidant to the liposomes containing the amphotericin B before adding the calcium. 
     
     
         48 . The method of  claim 45 , farther comprising adjusting the pH of the geode liposomes of step (b) or the geode cochleate of step (c) to neutral. 
     
     
         49 . The method of  claim 45 , further comprising lyophilizing the geode cochleate.