EB Matrix Production from Fetal Tissues and its Use for Tissue Repair

Abstract

A method of performing and preserving a bioremodelable, biopolymer scaffold material by subjecting animal tissue to chemical and mechanical processing. In addition to skin tissue, another source of EBM is a blood vessel. EBM may be used for hernia repair, colon, rectal, vaginal and or urethral prolapse treatment; pelvic floor reconstruction; muscle flap reinforcement; lung tissue support; rotator cuff repair or replacement; periosteum replacement; dura repair; pericardial membrane repair; soft tissue augmentation; intervertebral disk repair; and periodontal repair. EBM may also be used as a urethral sling, laminectomy barrier or spinal fusion device.

1 . A method for producing and preserving a biopolymer scaffold material, comprising the steps of:
 a. harvesting tissue from an animal source;   b. optionally extracting growth and differentiation factors from said tissue;   c. inactivating infective agents of said tissue;   d. mechanically expressing undesirable components from said tissue;   e. delipidizing said tissue;   f. washing said tissue for removal of chemical residues;   g. optionally drying said tissue; and   h. optionally cross-linking said tissue.   
     
     
         2 .- 31 . (canceled)