Anti-Proliferative Effects of Palm Vegetation Liquor And Extracts Thereof In Preventing Pancreatic Cancer

Abstract

Present invention relates to a composition to inhibit cancer cell proliferation, wherein said composition comprises oil palm extracts. The composition is useful for prevention of pancreatic cancer by inhibiting clonogenicity, inducing apoptosis, regulating gene expression, inducing anti-invasive effect, and induces cell cycle arrest in S phase. Present invention also discloses a composition that inhibits NF-κB activity and reduces cell invasion, cell migration and metastasis. Present invention further discloses the use of therapeutically effective amount of a composition in inhibiting the growth of pancreatic cancer.

What is claimed is: 
     
         1 . A composition to inhibit pancreatic cancer cell proliferation and clonogenicity, wherein said composition comprises plant extracts. 
     
     
         2 . The composition as claimed in  claim 1 , wherein said plant extract is from oil palm. 
     
     
         3 . The composition as claimed in  claim 1 , wherein said plant extracts is from vegetation liquor. 
     
     
         4 . The composition as claimed in  claim 3 , wherein said vegetation liquor further comprising phenolics. 
     
     
         5 . The composition as claimed in  claim 1 , wherein said composition is useful for prevention of pancreatic cancer and diseases related thereto. 
     
     
         6 . The composition as claimed in  claim 1 , wherein said pancreatic cancer is from PANC-1 cell line. 
     
     
         7 . The composition as claimed in  claim 1 , wherein said pancreatic cancer is from BxPC-3 cell line. 
     
     
         8 . The composition as claimed in  claim 1 , wherein said composition induces apoptosis. 
     
     
         9 . The composition as claimed in  claim 1 , wherein said composition regulates gene expression. 
     
     
         10 . The composition as claimed in  claim 8 , wherein said apoptosis is associated with inhibiting cell survival proteins. 
     
     
         11 . The composition as claimed in  claim 8 , wherein said apoptosis is associated with inhibiting anti-apoptotic protein. 
     
     
         12 . The composition as claimed in  claim 11 , wherein said anti-apoptotic protein is Survivin. 
     
     
         13 . The composition as claimed in  claim 9 , wherein said gene expression regulated is associated with Bcl-2 family. 
     
     
         14 . The composition as claimed in  claim 9 , wherein said gene expression is associated with down-regulating Bcl-XL expression. 
     
     
         15 . The composition as claimed in  claim 8 , wherein said apoptosis is associated with increased expression of pro-apoptotic proteins. 
     
     
         16 . The composition as claimed in  claim 15 , wherein said pro-apoptotic proteins are cleaved caspase 3, caspase 9 or Poly (ADP-ribose) polymerase (PARP). 
     
     
         17 . The composition as claimed in  claim 1 , wherein said composition induces cell cycle arrest in S phase. 
     
     
         18 . The composition as claimed in  claim 1 , wherein said composition inhibits NF-κB activity. 
     
     
         19 . The composition as claimed in  claim 18 , wherein said NF-κB activity inhibition is via down-regulating p65 subunit activity. 
     
     
         20 . The composition as claimed in  claim 19 , wherein said down-regulating is associated with decreasing vascular endothelial growth factor (VEGF) gene expression. 
     
     
         21 . The composition as claimed in  claim 19 , wherein said down-regulating is associated with decreasing matrix metalloproteinase 9 (MMP9) gene expression. 
     
     
         22 . The composition as claimed in  claim 1 , wherein said composition reduces cell invasion. 
     
     
         23 . The composition as claimed in  claim 1 , wherein said composition reduces cell migration and metastasis. 
     
     
         24 . The composition as claimed in  claim 1 , further comprising compounds with pharmaceutically acceptable carriers. 
     
     
         25 . A method of using a therapeutically effective amount of a composition to inhibit pancreatic cancer cell proliferation and clonogenicity, wherein said composition comprises plant extracts, and wherein the method is used in the preparation of a medicament for preventing or inhibiting the growth of pancreatic cancer in an individual by administering to an individual in need thereof. 
     
     
         26 . The method as claimed in  claim 25 , wherein the composition is accompanied with or without conventional chemotherapy or radiation therapy or surgery, 
     
     
         27 . The method as claimed in  claim 25 , wherein the composition is administered orally or as a food supplement.