Maintenance medium for primate pluripotent stem cells

Abstract

Provided are a maintenance medium for primate pluripotent stem cells, and a method for preserving and a method for controlling proliferation of primate pluripotent stem cells using the medium. The maintenance medium for human pluripotent stem cells according to the present invention includes xylose as a saccharide.

What is claimed is: 
     
         1 . A maintenance medium for pluripotent stem cells, comprising xylose as a saccharide, which is substantially free of glucose. 
     
     
         2 . The maintenance medium for pluripotent stem cells according to  claim 1 , wherein the primate pluripotent stem cells are human pluripotent stem cells. 
     
     
         3 . The maintenance medium for pluripotent stem cells according to  claim 1 , wherein the primate pluripotent stem cells are human iPS cells. 
     
     
         4 . The maintenance medium for pluripotent stem cells according to  claim 1 , wherein the xylose concentration is 0.7 to 12.0 g/L at the final concentration. 
     
     
         5 . The maintenance medium for pluripotent stem cells according to  claim 1 , wherein the maintenance medium is a culture medium for human iPS cells containing xylose in place of glucose. 
     
     
         6 . The maintenance medium for pluripotent stem cells according to  claim 5 , wherein the basal medium of a culture medium for human iPS cells is a DMEM/F12 medium. 
     
     
         7 . A method for preserving primate pluripotent stem cells, which comprises maintaining primate pluripotent stem cells using a medium comprising xylose as a saccharide, which is substantially free of glucose. 
     
     
         8 . The method according to  claim 7 , wherein the primate pluripotent stem cells are human pluripotent stem cells. 
     
     
         9 . The method according to  claim 7 , wherein the primate pluripotent stem cells are human iPS cells. 
     
     
         10 . The method according to  claim 7 , wherein the xylose concentration is 0.7 to 12.0 g/L at the final concentration. 
     
     
         11 . The method according to  claim 7 , wherein the maintenance medium is a culture medium for human iPS cells containing xylose in place of glucose. 
     
     
         12 . The method according to  claim 11 , wherein the basal medium of a culture medium for human iPS cells is a DMEM/F12 medium. 
     
     
         13 . A method for controlling proliferation of primate pluripotent stem cells, which comprises inhibiting the proliferation of primate pluripotent stem cells using a medium comprising xylose as a saccharide, which is substantially free of glucose. 
     
     
         14 . The method according to  claim 13 , which further comprises promoting the proliferation of primate pluripotent stem cells using a medium comprising glucose. 
     
     
         15 . The method according to  claim 13 , wherein the primate pluripotent stem cells are human pluripotent stem cells. 
     
     
         16 . The method according to  claim 13 , wherein the primate pluripotent stem cells are human iPS cells. 
     
     
         17 . The method according to  claim 13 , wherein the xylose concentration is 0.7 to 12.0 g/L at the final concentration. 
     
     
         18 . The method according to  claim 13 , wherein the maintenance medium is a culture medium for human iPS cells containing xylose in place of glucose. 
     
     
         19 . The method according to  claim 18 , wherein the basal medium of a culture medium for human iPS cells is a DMEM/F12 medium.