Activated leukocyte composition and uses for wound healing

Abstract

Disclosed are therapeutic, blood-derived activated leukocyte compositions, methods of making them, and methods of using the compositions to repair or promote the prevention and healing of wounds.

1 - 44 . (canceled) 
     
     
         45 . A method for treating a wound comprising administering to the wound the composition comprising activated leukocytes, wherein the activated leukocytes are made by a method comprising:
 a) incubating human leukocytes for a period of time so that the leukocytes transition from a quiescent to a functionally active state;   b) subjecting the leukocytes to hypo-osmotic shock; and   c) adding to the leukocytes of step b) physiologically acceptable solution comprising inorganic ions, organic osmolytes, or some combination thereof, in an amount which restores isotonicity.   
     
     
         46 . The method of  claim 45 , wherein the wound is a decubital ulcer, a pressure ulcer, a lower extremity ulcer, a deep sternal wound, a post-operative wound, a refractory post-operative wound of the trunk area, a wound to the great saphenous vein following harvesting of the great saphenous vein, a venous ulcer, or an anal fissure. 
     
     
         47 . The method of  claim 46 , wherein the lower extremity ulcer is in a diabetic patient. 
     
     
         48 . The method of  claim 46 , wherein the wound is a venous ulcer, pressure ulcer, or post-operative ulcer. 
     
     
         49 . A method for treating a wound comprising administering to the wound the composition comprising activated leukocytes, wherein the activated leukocytes comprise:
 a) at least 95% viable leukocytes based on the total number of leukocyte cells in the activated leukocyte composition; and/or   b) at least 75% CD11b(+) granulocytes relative to the total granulocyte population in the activated leukocyte composition.   
     
     
         50 . The method of  claim 49 , wherein the wound is a decubital ulcer, a pressure ulcer, a lower extremity ulcer, a deep sternal wound, a post-operative wound, a refractory post-operative wound of the trunk area, a wound to the great saphenous vein following harvesting of the great saphenous vein, a venous ulcer, or an anal fissure. 
     
     
         51 . The method of  claim 50 , wherein the lower extremity ulcer is in a diabetic patient. 
     
     
         52 . The method of  claim 50 , wherein the wound is a venous ulcer, pressure ulcer, or post-operative ulcer. 
     
     
         53 . A method for treating a wound comprising administering to the wound the article of manufacture comprising an activated leukocyte composition and a dressing, wherein the activated leukocytes of the activated leukocyte composition comprise:
 a) at least 95% viable leukocytes based on the total number of leukocyte cells in the activated leukocyte composition; and/or   b) at least 75% CD11b(+) granulocytes relative to the total granulocyte population in the activated leukocyte composition.