Fluid associated with adult stem cells for medical, cosmetic, and veterinary use

Abstract

Apparatus and process to create, expand, and localize the population of stem cells in adult tissues by placing a foreign body either in omentum or in the subcutaneous tissue. The apparatus 1) traps the granulation fluid, cells a fluid rich in a myriad of growth factors, 2) mobilizes and concentrates free-floating stem cells in the granulation fluid and 3) stimulates the growth of a new tissue around the apparatus, which provides another source of stem cells. Stem cells obtained by this process can be used as freshly isolated or after culturing, which can be used for wound healing, treating diseases, and regenerating organs in animals and humans. The granulation fluid could substitute the stem cells in many of their applications and offers the convenience of an injectable medicine for treating animal and human diseases

What is claimed is: 
     
         1 . A method for producing fluid associated with adult stem cells, the method comprising:
 implanting a foreign body under the skin or in the omentum of a live mammal, to produce a granuloma structure comprising an encapsulating tissue, which forms in response to the foreign body, and a fluid containing cellular material, which collects within the granuloma structure;   isolating the fluid from the granuloma structure; and   optionally converting the fluid into a solid form, wherein the fluid so obtained contains albumin, globulins and at least 2 ng/mL of vascular endothelial growth factor (VEGF), and exceeds FBS in potency for promoting the growth of HK and primary mesangial cells in culture media.   
     
     
         2 . The method of  claim 1 , wherein isolating the fluid comprises removing from the granuloma structure the fluid containing cellular material, and removing the cellular material therefrom. 
     
     
         3 . The method of  claim 2 , wherein removing the cellular material comprises centrifugation. 
     
     
         4 . The method of  claim 1 , comprising converting the fluid into a solid form. 
     
     
         5 . The method of  claim 4 , wherein converting the fluid into a solid form comprises lyophilization. 
     
     
         6 . The method of  claim 4 , wherein isolating the fluid comprises removing from the granuloma structure the fluid containing cellular material, and removing the cellular material therefrom. 
     
     
         7 . The method of  claim 4 , wherein removing the cellular material comprises centrifugation. 
     
     
         8 . The method of  claim 1 , wherein the fluid is harvested from a pig, cow, horse, sheep, dog, rat, or rabbit. 
     
     
         9 . The method of  claim 1 , wherein the fluid is harvested from a horse. 
     
     
         10 . The method of  claim 2 , wherein the fluid is harvested from a pig, cow, horse, sheep, dog, rat, or rabbit. 
     
     
         11 . The method of  claim 2 , wherein the fluid is harvested from a horse. 
     
     
         12 . The method of  claim 3 , wherein the fluid is harvested from a pig, cow, horse, sheep, dog, rat, or rabbit. 
     
     
         13 . The method of  claim 3 , wherein the fluid is harvested from a horse. 
     
     
         14 . The method of  claim 4 , wherein the fluid is harvested from a pig, cow, horse, sheep, dog, rat, or rabbit. 
     
     
         15 . The method of  claim 4 , wherein the fluid is harvested from a horse. 
     
     
         16 . The method of  claim 5 , wherein the fluid is harvested from a pig, cow, horse, sheep, dog, rat, or rabbit. 
     
     
         17 . The method of  claim 5 , wherein the fluid is harvested from a horse. 
     
     
         18 . The method of  claim 6 , wherein the fluid is harvested from a pig, cow, horse, sheep, dog, rat, or rabbit. 
     
     
         19 . The method of  claim 6 , wherein the fluid is harvested from a horse. 
     
     
         20 . The method of  claim 7 , wherein the fluid is harvested from a pig, cow, horse, sheep, dog, rat, or rabbit. 
     
     
         21 . The method of  claim 7 , wherein the fluid is harvested from a horse. 
     
     
         22 . The product produced according to  claim 1 . 
     
     
         23 . The product produced according to  claim 2 . 
     
     
         24 . The product produced according to  claim 3 . 
     
     
         25 . The product produced according to  claim 4 . 
     
     
         26 . The product produced according to  claim 5 . 
     
     
         27 . The product produced according to  claim 6 . 
     
     
         28 . The product produced according to  claim 7 . 
     
     
         29 . The product produced according to  claim 8 . 
     
     
         30 . The product produced according to  claim 9 . 
     
     
         31 . The product produced according to  claim 10 . 
     
     
         32 . The product produced according to  claim 11 . 
     
     
         33 . The product produced according to  claim 12 . 
     
     
         34 . The product produced according to  claim 13 . 
     
     
         35 . The product produced according to  claim 14 . 
     
     
         36 . The product produced according to  claim 15 . 
     
     
         37 . The product produced according to  claim 16 . 
     
     
         38 . The product produced according to  claim 17 . 
     
     
         39 . The product produced according to  claim 18 . 
     
     
         40 . The product produced according to  claim 19 . 
     
     
         41 . The product produced according to  claim 20 . 
     
     
         42 . The product produced according to  claim 21 .