Microfluidic Droplet Queuing Network

Abstract

A multi-port liquid bridge ( 1 ) adds aqueous phase droplets ( 10 ) in an enveloping oil phase carrier liquid ( 11 ) to a draft channel ( 4, 6 ). A chamber ( 3 ) links four ports, and it is permanently full of oil ( 11 ) when in use. Oil phase is fed in a draft flow from an inlet port ( 4 ) and exits through a draft exit port ( 6 ) and a compensating flow port ( 7 ). The oil carrier and the sample droplets ( 3 ) (“aqueous phase”) flow through the inlet port ( 5 ) with an equivalent fluid flow subtracted through the compensating port ( 7 ). The ports of the bridge ( 1 ) are formed by the ends of capillaries help in position in plastics housings. The phases are density matched to create an environment where gravitational forces are negligible. This results in droplets ( 10 ) adopting spherical forms when suspended from capillary tube tips. Furthermore, the equality of mass flow is equal to the equality of volume flow. The phase of the inlet flow from the droplet inlet port ( 5 ) and the draft inlet port ( 4 ) is used to determine the outlet port ( 6 ) flow phase.

1 . A microfluidic network for queuing a sequence of droplets in an immiscible carrier liquid, the network comprising:
 a draft conduit for flow of droplets with a carrier liquid;   at least one liquid bridge in the draft conduit, the bridge having a chamber in which there is a draft inlet, a draft outlet, an inlet port, and a compensation port;   wherein the inlet port is positioned for delivery of liquid to be queued into the draft conduit so that said liquid flows from the bridge in the carrier liquid in the draft conduit, and   wherein the compensation port is positioned for withdrawal of carrier liquid to compensate for liquid added to the draft conduit via the inlet port.   
     
     
         2 . A network as claimed in  claim 1 , wherein the compensation port is configured to provide a uniform target flow in the draft conduit. 
     
     
         3 . A network as claimed in  claim 1 , wherein the draft conduit comprises a plurality of bridges and a liquid supply is connected to the inlet port of each bridge. 
     
     
         4 . A network as claimed in  claim 1 , wherein the inlet port and the draft conduit are co-planar. 
     
     
         5 . A network as claimed in  claim 4 , wherein the compensation port is at an angle to the plane of the inlet port and the draft conduit. 
     
     
         6 . A network as claimed in  claim 5 , wherein the compensation port is at an angle of substantially 90° to the plane of the draft conduit and the inlet port. 
     
     
         7 . A network as claimed in  claim 1 , wherein the draft conduit inlet and an outlet to the bridge are at approximately 120° to each other and the inlet port is in-plane with the draft conduit and at an angular separation of 120° from each of the draft inlet and outlet. 
     
     
         8 . A network as claimed in  claim 1 , comprising a plurality of draft conduits in parallel. 
     
     
         9 . (canceled) 
     
     
         10 . (canceled) 
     
     
         11 . (canceled) 
     
     
         12 . A network as claimed in  claim 1 , further comprising a segmenter for segmenting a large droplet or a stream into droplets and for delivering said droplets to the inlet port of the bridge. 
     
     
         13 . A network as claimed in  claim 12 , wherein the segmenter comprises a bridge having a chamber with an inlet and an outlet, and said inlet and outlet are configured so that a droplet temporarily adheres to the inlet and transfers to the outlet when it becomes unstable. 
     
     
         14 . A network as claimed in  claim 13 , wherein the segmenter comprises a chamber for containing carrier liquid in the space between the inlet and the outlet. 
     
     
         15 . A network as claimed in  claim 1 , wherein the network comprises a plurality of bridges in the draft conduit, at least one of said bridges being a mixing bridge downstream of at least one other bridge, the mixing bridge comprising means for mixing a droplet with a droplet flowing in the draft conduit. 
     
     
         16 . A network as claimed in  claim 15 , wherein the mixing bridge comprises an inlet port for an added droplet, configured for formation of droplets within its chamber, for contact and mixing of said droplets, and for transfer of the mixed droplet to the draft outlet. 
     
     
         17 . A network as claimed in  claim 16 , wherein the mixing bridge chamber is configured to fill with carrier liquid to surround the droplets in the chamber. 
     
     
         18 . A network as claimed in  claim 3 , wherein said supply comprises a well and a manifold for delivering droplets from the well to the plurality of bridges. 
     
     
         19 . A network as claimed in  claim 18 , wherein the network further comprises an infusion pump for delivering carrier liquid to the manifold. 
     
     
         20 . A network as claimed in  claim 18 , wherein the plurality of bridges and the draft conduit are arranged in an array and there is a well adjacent each bridge. 
     
     
         21 . A network as claimed in  claim 20 , wherein said wells are arranged in a pattern of an assay well plate. 
     
     
         22 . A network as claimed in  claim 18 , wherein there are a plurality of wells and associated manifolds, and they are arranged for delivery of droplets of different types to the bridges to achieve a serial flow of droplets of different types in the draft conduit. 
     
     
         23 . A network as claimed in  claim 22 , wherein the bridges are arranged so that droplets are added simultaneously at spaced-apart locations along the draft conduit. 
     
     
         24 . (canceled) 
     
     
         25 . (canceled) 
     
     
         26 . (canceled) 
     
     
         27 . (canceled)