Microorganisms for Producing Volatile Organic Compounds

Abstract

A method for producing at least one compound selected from the group consisting of 1,8-cineole, and 1-methyl-1,4-cyclohexadiene is also described. The method includes culturing a microorganism on or within a culturing media in a container under conditions sufficient for producing the at least one compound.

1 .- 21 . (canceled) 
     
     
         22 . An isolated fungus having the imperfect stage of  Nodulisporium , wherein the fungus produces at least one compound selected from the group consisting of 1,8-cineole and 1-methyl-1,4-cyclohexadiene. 
     
     
         23 . The isolated fungus of  claim 22 , wherein the fungus is from the genus  Nodulisporium.    
     
     
         24 . The isolated fungus of  claim 22 , wherein the fungus is from the genus  Hypoxylon.    
     
     
         25 . The isolated fungus of  claim 22 , wherein the fungus is from the genus  Annulohypoxylon.    
     
     
         26 . The isolated fungus of  claim 22 , wherein the fungus is from the genus  Daldinia.    
     
     
         27 . The isolated fungus of  claim 22 , wherein the fungus is from the genus  Xylaria.    
     
     
         28 . The isolated fungus of  claim 22 , wherein the fungus is serially propagated. 
     
     
         29 . The isolated fungus of  claim 22 , wherein the fungus is grown on or in a high-starch substrate. 
     
     
         30 . The isolated fungus of  claim 22 , where the fungus is grown in a liquid medium. 
     
     
         31 . The isolated fungus of  claim 22 , wherein the fungus is grown on a solid medium. 
     
     
         32 . The isolated fungus of  claim 22 , wherein the at least one compound is isolated from the culturing media. 
     
     
         33 . The isolated fungus of  claim 22 , wherein the at least one compound is isolated from a vapor produced within a culturing container containing the culturing media. 
     
     
         34 . The isolated fungus of  claim 29 , the wherein high starch substrate is cellulose. 
     
     
         35 . The isolated fungus of  claim 32 , wherein the culturing media comprises at least one substrate selected from the group consisting of yeast, peptone, starch, glucose, cellobiose, potato dextrose (PDA), oatmeal agar, and instant mashed potatoes (MP). 
     
     
         36 . The isolated fungus of  claim 35 , wherein the culturing media is potato dextrose (PDA). 
     
     
         37 . The isolated fungus of  claim 22 , wherein the fungus is selected from the group consisting of Ni25-2A, Th-9, Fl-9, and PC-37-24. 
     
     
         38 . The isolated fungus of  claim 22 , wherein the fungus produces 1,8-cineole. 
     
     
         39 . A biofuel comprising 1,8-cineole produced by an isolated endophytic fungus capable of demonstrating the imperfect stage of  Nodulisporium.    
     
     
         40 . The biofuel of  claim 39 , wherein the fungus is grown on a solid medium. 
     
     
         41 . The biofuel of  claim 40 , wherein the solid medium is a biomass.