Intergeneric hybrid plants and methods for production thereof

Abstract

Methods for the production of an intergeneric hybrid plants and plants produced thereby. In certain aspects, intergeneric hybrid plants are produced by crossing a sorghum parent plant comprising a mutant sorghum iap allele with a second moncot plant. Methods for the use of such plants and products obtained therefrom are also provided.

1 . An intergeneric hybrid plant or part thereof produced by crossing a  sorghum  parent plant with a  Saccharum  parent plant, wherein the  sorghum  parent plant is homozygous for a recessive iap allele. 
     
     
         2 . The plant of  claim 1 , wherein the  sorghum  parent plant is a  Sorghum bicolor  plant. 
     
     
         3 . The plant of  claim 1 , wherein the  sorghum  parent plant is male sterile. 
     
     
         4 . The plant of  claim 3 , wherein the  sorghum  parent plant is a plant of  sorghum  line Tx3361. 
     
     
         5 . The plant of  claim 1 , wherein the  Saccharum  parent plant is a  Saccharum spontaneum, S. officinarum  or a  Saccharum officinarum×Saccharum spontaneum  hybrid plant. 
     
     
         6 . The plant of  claim 5 , wherein the  Saccharum  parent plant is sugarcane variety L06-024, HoCP05-904 or Ho06-562. 
     
     
         7 . The plant of  claim 1 , further comprising a transgene, wherein the transgene confers disease resistance, insect resistance, herbicide resistance, drought tolerance, salt tolerance, male sterility or enhanced sugar content. 
     
     
         8 - 9 . (canceled) 
     
     
         10 . The plant part of  claim 1 , further defined as a protoplast, cell, gamete, meristem, root, pistil, anther, flower, seed, embryo, stalk, petiole, or a 2n plant gamete. 
     
     
         11 . (canceled) 
     
     
         12 . A seed that produces a plant of  claim 1 . 
     
     
         13 . The seed of  claim 12 , further defined as coated with an agent comprising a pesticide, a fungicide, a nutrient or a water or temperature-sensitive polymer or an agent to improve seed mechanical handling properties, seed germination, seedling establishment or growth. 
     
     
         14 . A commercial product obtained from a plant according to  claim 1 , wherein the commercial product is a fermentable biofuel feedstock, cane juice, molasses, bagasse, ethanol, biodiesel or sugar. 
     
     
         15 . (canceled) 
     
     
         16 . A progeny plant of the plant according to  claim 1 , wherein the progeny comprises at least a first chromosome or segment thereof from the  sorghum  parent plant and least a first chromosome or segment thereof from the  Saccharum  parent plant. 
     
     
         17 . A tissue culture of regenerable cells of the plant of  claim 1 , wherein the regenerable cells are from embryos, meristematic cells, pollen, leaves, roots, root tips, anther, pistil, flower, seed, boll or stem. 
     
     
         18 . (canceled) 
     
     
         19 . A plant regenerated from the tissue culture of  claim 17 . 
     
     
         20 . A method for producing a commercial product comprising obtaining a plant of  claim 1  or a part thereof and producing a commercial product therefrom, wherein the commercial product is a fermentable biofuel feedstock, cane juice, molasses, bagasse, ethanol, biodiesel or sugar. 
     
     
         21 . (canceled) 
     
     
         22 . A  sorghum  plant comprising an allele conferring male sterility, wherein the plant is homozygous for a recessive iap allele. 
     
     
         23 . The  sorghum  plant of  claim 22 , wherein the allele is ms3. 
     
     
         24 . A seed that produces the  sorghum  plant of  claim 22 . 
     
     
         25 - 28 . (canceled) 
     
     
         29 . An intergeneric hybrid plant or part thereof produced by crossing a  sorghum  parent plant with a second monocot plant from a different genus, wherein the  sorghum  parent plant is homozygous for a recessive iap allele. 
     
     
         30 . The plant of  claim 29 , wherein the  sorghum  parent plant is a  Sorghum bicolor  plant. 
     
     
         31 . The plant of  claim 29 , wherein the  sorghum  parent plant comprises male sterility. 
     
     
         32 . The plant of  claim 31 , wherein the  sorghum  parent plant is a plant of  sorghum  line Tx3361. 
     
     
         33 . The plant of  claim 29 , wherein the second monocot plant is a plant from the family Poaceae. 
     
     
         34 . The plant of  claim 29 , wherein the second monocot plant is a  Saccharum, Miscanthus , a  Saccharum×Miscanthus  hybrid,  Erianthus, Sorghastrum, Panicum, Pennisetum  or  Zea  plant. 
     
     
         35 . The plant of  claim 29 , wherein the second monocot plant is a  Panicum virgatum, Pennisetum purpureum, Pennisetum ciliare, Pennisetum glaucum, Andropogon gerardii, Andropogon hallii, Schizachyrium scoparium, Sorghastrum nutans, Arundo donax, Tripsicum dactyloides, Sporobolus airiodes, Miscanthus floridulus, Miscanthus sinensis, Zea mays, Zea nicaraguensis, Zea perennis  or  Zea diploperennis  plant. 
     
     
         36 . The plant of  claim 29 , further comprising a transgene, wherein the transgene confers disease resistance, insect resistance, herbicide resistance, drought tolerance, salt tolerance, male sterility or enhanced sugar content. 
     
     
         37 - 38 . (canceled) 
     
     
         39 . The plant part of  claim 29 , further defined as a protoplast, cell, gamete, meristem, root, pistil, anther, flower, seed, embryo, stalk, petiole, or a 2n plant gamete. 
     
     
         40 . (canceled) 
     
     
         41 . A seed that produces a plant of  claim 29 . 
     
     
         42 . A seed of  claim 41 , further defined as coated with an agent comprising a pesticide, a fungicide, a nutrient or a water or temperature-sensitive polymer or an agent to improve seed mechanical handling properties, seed germination, seedling establishment or growth. 
     
     
         43 . A commercial product obtained from a plant according to  claim 29 , wherein the commercial product is a fermentable biofuel feedstock, cane juice, molasses, bagasse, ethanol, biodiesel or sugar. 
     
     
         44 . (canceled) 
     
     
         45 . A progeny plant of the plant according to  claim 29 , wherein the progeny comprises at least a first chromosome or segment thereof from the  sorghum  parent plant and least a first chromosome or segment thereof from the second monocot parent plant. 
     
     
         46 . A tissue culture of regenerable cells of the plant of  claim 29 , wherein the regenerable cells are from embryos, meristematic cells, pollen, leaves, roots, root tips, anther, pistil, flower, seed, boll or stem. 
     
     
         47 . (canceled) 
     
     
         48 . A plant regenerated from the tissue culture of  claim 46 . 
     
     
         49 . A method for producing a commercial product comprising obtaining a plant of  claim 29  or a part thereof and producing a commercial product therefrom, wherein the commercial product is a fermentable biofuel feedstock, cane juice, molasses, bagasse, ethanol, biodiesel or sugar. 
     
     
         50 . (canceled) 
     
     
         51 . A method for producing an intergeneric hybrid embryo or seed, the method comprising crossing a  sorghum  parent plant with a second monocot parent plant, wherein the  sorghum  plant is homozygous for a recessive  sorghum  sap allele and is used as a female parent. 
     
     
         52 . The method of  claim 51 , comprising:
 (i) rescuing an embryo resulting from the crossing;   (ii) growing an embryo resulting from the cross to produce an intergeneric hybrid plant; or   (iii) wherein the embryo is comprised in a seed having a functional endosperm.   
     
     
         53 - 58 . (canceled) 
     
     
         59 . The method of  claim 51 , wherein the monocot parent plant is selected from the family Poaceae, or from the group consisting of  Saccharum, Miscanthus, Erianthus, Zea, Sorghastrum, Panicum  and  Pennisetum;    or wherein the monocot parent plant is a  Panicum virgatum, Pennisetum purpureum, Pennisetum ciliare, Pennisetum glaucum, Andropogon gerardii, Andropogon hallii, Schizachvrium scoparium, Sorghastrum nutans, Arundo donax, Tripsicum dactyloides, Sporobolus airiodes, Miscanthus floridulus, Miscanthus sinensis, Zea mays, Zea nicaraguensis Zea perennis  or  Zea diploperennis  plant.   
     
     
         60 - 65 . (canceled) 
     
     
         66 . An intergeneric hybrid seed, plant or part thereof produced according to the method of  claim 51 . 
     
     
         67 . A progeny intergeneric hybrid plant of a plant according to  claim 52  wherein the progeny comprises at least a first chromosome or segment thereof from the  sorghum  parent plant and least a first chromosome or segment thereof from the second monocot parent plant.