Detecting nucleic acids

Abstract

Described herein are methods and compositions for detecting, amplifying and labeling targeted nucleic acids, including microRNAs.

1 - 25 . (canceled) 
     
     
         26 . A biochip comprising:
 (a) a plurality of negative probes;   (b) a plurality of positive probes;   (c) a plurality of spike probes; and   (d) a plurality of test probes;   
       wherein said test probe comprises (i) a nucleic acid substantially complementary to a miRNA; and (ii) a linker; and wherein the test probe comprises 40 to 60 nucleotides. 
     
     
         27 . The biochip of  claim 26 , wherein a negative probe comprises a nucleic acid substantially complementary to a miRNA*. 
     
     
         28 . The biochip of  claim 27 , wherein the sequence of the nucleic acid is selected from the group consisting of any one of SEQ ID NOS: 20863-20925. 
     
     
         29 . The biochip of  claim 26 , wherein a positive probe comprises a nucleic acid substantially complementary to a small RNA. 
     
     
         30 . The biochip of  claim 29 , wherein the sequence of the nucleic acid is selected from the group consisting of any one of SEQ ID NOS: 20926-20937. 
     
     
         31 . The biochip of  claim 26 , wherein a spike probe comprises a nucleic acid with a sequence that is not complementary to any sequence of 60 nucleotides in a genome. 
     
     
         32 . The biochip of  claim 31 , wherein the genome is selected from the group consisting of human, rat, virus and mouse. 
     
     
         33 . The biochip of  claim 31 , wherein the sequence of the nucleic acid is selected from the group consisting of any one of SEQ ID NOS: 20938-20951. 
     
     
         34 . (canceled) 
     
     
         35 . The biochip of  claim 26 , wherein said test probe comprises a nucleic acid comprising 16 to 29 nucleotides. 
     
     
         36 . The biochip of  claim 35 , wherein the sequence of the nucleic acid is selected from the group consisting of any one of SEQ ID NOS: 1-4167. 
     
     
         37 . The biochip of  claim 26 , wherein said linker comprises a nucleic acid selected from the group consisting of any one of SEQ ID NOS: 20952-21063. 
     
     
         38 - 58 . (canceled)