Fluidic Interfacing System and Assembly

Abstract

A fluidic assay system assembly comprising: (a) A disposable fluidic cartridge ( 1 ) comprising at least one reaction chamber ( 3 ) connected to a network of fluidic channels ( 2 ) with at least one inlet channel and one outlet channel. The said inlet and outlet channels end at the down side of the fluidic cartridge with at least two connecting pores ( 4 ), ( 4′ ); (b) A disposable vessel ( 5 ) comprising a connection tube 22 immersed in a sample container ( 6 ) and ended at the cap of the vessel with an external connection pore ( 7 ) ; (c) A fluidic manifold ( 8 ) that is interdependent with the bulk system ( 12 ) comprising a fluidic network connected ( 9 ) to active fluidic parts ( 10 ), ( 11 ). The said channel network ends at the top side of the fluidic manifold with at least one connecting pore ( 13 ). Wherein the first and the second pores of the fluidic cartridge are interfaced by direct physical contact with the sample container and the manifold pores, respectively.

1 .- 11 . (canceled) 
     
     
         12 . A fluidic cartridge used for extracting target biomolecules or particles from a crude sample, the cartridge comprising:
 a. at least one structure top layer containing:
 i. a reaction chamber with a solid support that is designed to capture the said target biomolecules, 
 ii. a first inlet and outlet channels that are in fluid communication with the said reaction chamber and that will be used to bring the sample it and out the reaction chamber, and 
 iii. a second inlet and outlet channels connected to the said reaction chamber and that will be used for eluting the purified biomolecules 
 wherein the second inlet and outlet channels are diverging branch of the first inlet and outlet channels. 
   
     
     
         13 . The fluidic cartridge according to  claim 12 , wherein the solid support is constituted by magnetic particles. 
     
     
         14 . (canceled) 
     
     
         15 . The fluidic cartridge according to  claim 12 , wherein the said first inlet channel is in fluid communication with the sample vessel. 
     
     
         16 . The fluidic cartridge according to  claim 12 , wherein the said second inlet channel is in fluid communication with a recovery sample vessel. 
     
     
         17 . The fluidic cartridge according to  claim 12 , wherein the reaction chamber is further in fluid communication with a fluidic network channel that brings reagents in the said reaction chamber. 
     
     
         18 . The fluidic cartridge according to  claim 12 , wherein the cartridge further comprises a closing down layer composed from a flexible material and comprising connection pores associated to the ends of said inlet and outlet channels. 
     
     
         19 . The fluidic cartridge according to  claim 15 , wherein in processing, the sample is aspirated from the sample vessel, in fluid communication with the first inlet channel, into the reaction chamber through the first outlet channel. 
     
     
         20 . The fluidic cartridge according to  claim 16 , wherein in processing, the target biomolecules or particles are eluted into the recovery sample vessel, in fluid communication with the second outlet channel, from the reaction chamber by pouching air through second inlet channel. 
     
     
         21 . The fluidic cartridge according to  claim 12 , which comprises at least one optical sensor positioned in one side of said reaction chamber. 
     
     
         22 . The fluidic cartridge according to  claim 13 , wherein the magnetic particles are manipulated and mixed using at least two electromagnetic poles face each other across the reaction chamber, and wherein the said magnetic poles are actuated by:
 a) applying magnetic field sequences having polarity and intensity that vary in time from the electromagnetic poles, wherein said magnetic field sequences break or inhibit the particle aggregates and maintain the particles in suspension as a fog of particles in relative dynamic movement; and   b) combining the magnetic fields from different magnetic poles in a sequence to induce displacement of the fog of particles across the reaction chamber, wherein the fog of particles occupies substantially the whole reaction chamber volume.