Endolysin obpgplys

Abstract

The present invention relates to a polypeptide with an amino acid sequence according to SEQ ID NO: 1 and fragments or derivatives thereof. The present invention further relates to fusion proteins comprising said polypeptide and an additional peptide stretch fused to said polypeptide at the N- or C-terminus. Moreover, the present invention relates to nucleic acid molecules encoding said polypeptide or fusion protein, vectors comprising said nucleic acid molecules and host cells comprising either said nucleic acid molecules or said vectors. In addition, the present invention relates to said polypeptide or fusion protein for use as a medicament, in particular for the treatment or prevention of Gram-negative bacterial infections, as diagnostic means, as cosmetic substance or as sanitizing agent. The present invention also relates to the use of said polypeptide or fusion protein for the treatment or prevention of Gram-negative bacterial contamination of foodstuff, of food processing equipment, of food processing plants, of surfaces coming into contact with foodstuff, of medical devices, of surfaces in hospitals and surgeries. Furthermore, the present invention relates to a pharmaceutical composition comprising said polypeptide or fusion protein.

1 - 19 . (canceled) 
     
     
         20 . A method of disinfecting an environment comprising contacting that environment with a fusion polypeptide comprising (i) the amino acid sequence of SEQ ID NO: 1, or a fragment thereof, wherein the fragment comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 4 and 5, or comprises the amino acid sequences of SEQ ID NO: 4 and 5, and (ii) a heterologous polypeptide. 
     
     
         21 . A method of disinfecting an environment comprising contacting that environment with a fusion protein comprising (i) a polypeptide comprising the amino acid sequence of SEQ ID NO: 1, or a fragment thereof, wherein the fragment comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 4 and 5, or comprises the amino acid sequences of SEQ ID NO: 4 and 5, and (ii) a heterologous peptide fused to said polypeptide at the N- or C-terminus, wherein said peptide stretch is a cationic peptide, polycationic peptide, amphipathic peptide, sushi peptide, defensin, hydrophobic peptide and/or an antimicrobial peptide. 
     
     
         22 . A method treating or preventing a Gram-negative bacterial infection comprising administering to a human or animal subject a fusion polypeptide comprising (i) the amino acid sequence of SEQ ID NO: 1, or a fragment thereof, wherein the fragment comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 4 and 5, or comprises the amino acid sequences of SEQ ID NO: 4 and 5, and (ii) a heterologous polypeptide. 
     
     
         23 . A method treating or preventing a Gram-negative bacterial infection comprising administering to a human or animal subject a fusion protein comprising (i) a polypeptide comprising the amino acid sequence of SEQ ID NO: 1, or a fragment thereof, wherein the fragment comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 4 and 5, or comprises the amino acid sequences of SEQ ID NO: 4 and 5, and (ii) a heterologous peptide fused to said polypeptide at the N- or C-terminus, wherein said peptide stretch is a cationic peptide, polycationic peptide, amphipathic peptide, sushi peptide, defensin, hydrophobic peptide and/or an antimicrobial peptide. 
     
     
         24 . A method of treating or preventing a Gram-negative bacterial contamination of a foodstuff, a food processing equipment, a food processing plant, a surface coming into contact with foodstuff, a medical device, a surface in a medical facility or a surgical environment comprising contacting said foodstuff, equipment, plant, surface, medical device, facility or environment with a fusion polypeptide comprising (i) the amino acid sequence of SEQ ID NO: 1, or a fragment thereof, wherein the fragment comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 4 and 5, or comprises the amino acid sequences of SEQ ID NO: 4 and 5, and (ii) a heterologous polypeptide. 
     
     
         25 . A method of treating or preventing a Gram-negative bacterial contamination of a foodstuff, a food processing equipment, a food processing plant, a surface coming into contact with foodstuff, a medical device, a surface in a medical facility or a surgical environment comprising contacting said foodstuff, equipment, plant, surface, medical device, facility or environment with a fusion protein comprising (i) a polypeptide comprising the amino acid sequence of SEQ ID NO: 1, or a fragment thereof, wherein the fragment comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 4 and 5, or comprises the amino acid sequences of SEQ ID NO: 4 and 5, and (ii) a heterologous peptide fused to said polypeptide at the N- or C-terminus, wherein said peptide stretch is a cationic peptide, polycationic peptide, amphipathic peptide, sushi peptide, defensin, hydrophobic peptide and/or an antimicrobial peptide. 
     
     
         26 . A method diagnosing a Gram-negative bacterial infection comprising contacting a sample with a fusion polypeptide comprising (i) the amino acid sequence of SEQ ID NO: 1, or a fragment thereof, wherein the fragment comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 4 and 5, or comprises the amino acid sequences of SEQ ID NO: 4 and 5, and (ii) a heterologous polypeptide. 
     
     
         27 . A method of diagnosing a Gram-negative bacterial infection comprising contacting a sample with a fusion protein comprising (i) a polypeptide comprising the amino acid sequence of SEQ ID NO: 1, or a fragment thereof, wherein the fragment comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 4 and 5, or comprises the amino acid sequences of SEQ ID NO: 4 and 5, and (ii) a heterologous peptide fused to said polypeptide at the N- or C-terminus, wherein said peptide stretch is a cationic peptide, polycationic peptide, amphipathic peptide, sushi peptide, defensin, hydrophobic peptide and/or an antimicrobial peptide.