Methods and Compositions for Phototransfer

Abstract

Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.

1 . A method for binding a binding partner of a binding agent, comprising:
 a) providing: i) a micro-column comprising a plurality of binding agents attached to a surface through a photocleavable linker, ii) a source of electromagnetic radiation;   b) contacting said binding agents with a sample, said sample comprising a binding partner to said binding agents, under conditions such that at least a portion of said binding agents bind said binding partner so as to make a complex in said micro-column; and c) illuminating said complex in said micro-column with radiation from said radiation source under conditions such that said complex is photocleaved from said surface.   
     
     
         2 . The method of  claim 1 , wherein said surface is a bead surface. 
     
     
         3 . The method of  claim 1 , wherein said sample is a body fluid from a mammal. 
     
     
         4 . The method of  claim 3 , wherein said fluid is selected from the group consisting of blood, serum or plasma. 
     
     
         5 . The method of  claim 1 , wherein said binding partner is a peptide or protein. 
     
     
         6 . The method of  claim 1 , wherein said micro-column comprises a pipette tip. 
     
     
         7 . The method of  claim 6 , wherein said pipette tip has a 10 μL volume. 
     
     
         8 . The method of  claim 1 , wherein said binding agent is an antibody or aptamer. 
     
     
         9 . The method of  claim 1 , wherein said illuminating comprises exposing the micro-column to near-UV light. 
     
     
         10 . A method for binding a binding partner of a binding agent, comprising:
 a) providing: i) a micro-column comprising a plurality of binding agents attached to microbeads through a photocleavable linker; ii) a source of electromagnetic radiation;   b) contacting said binding agents with a sample, said sample comprising a binding partner to said binding agents, under conditions such that at least a portion of said binding agents bind said binding partner so as to make a complex in said micro-column; and c) illuminating said complex in said micro-column with radiation from said radiation source under conditions such that said complex is photocleaved from said microbeads.   
     
     
         11 . The method of  claim 10 , wherein said microbeads are agarose beads. 
     
     
         12 . The method of  claim 10 , wherein said microbeads are prevented from flowing out of the micro-column. 
     
     
         13 . The method of  claim 10 , wherein said sample is a body fluid from a mammal. 
     
     
         14 . The method of  claim 13 , wherein said fluid is selected from the group consisting of blood, serum or plasma. 
     
     
         15 . The method of  claim 10 , wherein said binding partner is a peptide or protein. 
     
     
         16 . The method of  claim 10 , wherein said micro-column comprises a pipette tip. 
     
     
         17 . The method of  claim 16 , wherein said pipette tip has a 10 μL volume. 
     
     
         18 . The method of  claim 10 , wherein said binding agent is an antibody or aptamer. 
     
     
         19 . The method of  claim 10 , wherein said illuminating comprises exposing the micro-column to near-UV light.