Applications of surfactin in emulsifying composition and thereof

Abstract

Surfactin is a biosurfactant produced by Bacillus subtilis and said surfactin is a natural cycloaliphatic peptide comprising a ring structure made of 7 amino acids, and said surfactin has various functions including anti-aging, anti-wrinkle, increasing skin penetration of cosmetic products (skin penetration agent), foaming agent and emulsifier.

What is claimed is: 
     
         1 . A method for preparation of a composition to improve skin penetration by using surfactin, wherein the composition comprises surfactin and pharmaceutically acceptable vehicles, excipients, diluents and adjuvants. 
     
     
         2 . The method as recited in  claim 1 , wherein the surfactin is a cycloaliphatic peptide comprising a hepapeptide (L)Glu-(L)Leu-(D)Leu-(L)Val-(L)Asp-(D)Leu-(L)Leu linked to a β-hydroxy fatty acid, and the distribution of the fatty acids at the fatty acid end are as follows:
 (1) iso-C13>3%; 
 (2) C13>0.65%; 
 (3) iso-C14>17%; 
 (4) C14<41%; and 
 (5) iso-C15<11%. 
 
     
     
         3 . The method as recited in  claim 2 , wherein the distribution of the fatty acids at the fatty acid end of the surfactin are as follows:
 (1) iso-C13>10%;   (2) C13>25%;   (3) iso-C14>35%;   (4) C14<25%; and   (5) iso-C15<3%.   
     
     
         4 . The method as recited in  claim 3 , wherein the distribution of the fatty acids at the fatty acid end of the surfactin are as follows: (1) iso-C 13=11%; (2) C13=26%; (3) iso-C 14=37%; (4) C14=24%; and (5) iso-C15=2%. 
     
     
         5 . The method as recited in  claim 1 , wherein the molecular weight of the surfactin is 1022 or 1036 Da. 
     
     
         6 . The method as recited in  claim 1 , wherein the surfactin comprises its isomers. 
     
     
         7 . The method as recited in  claim 1 , wherein the composition further comprises at least one of the following ingredients: alcohols, esters, complex polysaccharides, nut oils, and vitamins. 
     
     
         8 . The method as recited in  claim 7 , wherein the alcohol comprises at least one of the following: C16-18 alcohols, butanediol, pentanediol, octanediol, glycerin, hexadecanol, stearyl alcohol, 1-Docosanol and propylene glycol. 
     
     
         9 . The method as recited in  claim 7 , wherein the esters comprise at least one of the following: OLIVEM 1000, glycerol monostearate (GSM), isopropyl myristate (IPM), isopropyl palmitate (IPP) and triglycerides. 
     
     
         10 . The method as recited in  claim 7 , wherein the complex polysaccharides comprise at least one of the following: xanthan gum,  Tremella fuciformis  polysaccharides, dextran polysaccharides, and  Folium sennae  seed polysaccharides. 
     
     
         11 . The method as recited in  claim 7 , wherein the nut oil comprises at least one of the following: Argan oil, Kukui nut oil, avocado oil, wheat germ oil, and olive oil. 
     
     
         12 . The method as recited in  claim 7 , wherein the vitamins comprise at least one of the following: vitamin A, vitamin B, vitamin C, vitamin E, vitamin F, and vitamin K. 
     
     
         13 . The method as recited in  claim 1 , wherein the composition is used to promote skin penetration of cosmetic ingredients. 
     
     
         14 . The method as recited in  claim 13 , wherein the cosmetic ingredient is dexamethasone. 
     
     
         15 . The method as recited in  claim 13 , wherein the cosmetic ingredient is hyaluronic acid. 
     
     
         16 . The method as recited in  claim 13 , wherein the cosmetic ingredient is gamma-polyglutamic acid. 
     
     
         17 . The method as recited in  claim 13 , wherein the cosmetic ingredient is gold-nanoparticles. 
     
     
         18 . A method for preparation of an emulsifying composition using surfactin, wherein the composition comprises surfactin and pharmaceutically acceptable vehicles, excipients, diluents and adjuvants. 
     
     
         19 . The method as recited in  claim 18 , wherein the surfactin is a cycloaliphatic peptide comprising a hepapeptide (L)Glu-(L)Leu-(D)Leu-(L)Val-(L)Asp-(D)Leu-(L)Leu linked to a β-hydroxy fatty acid, and the distribution of the fatty acids at the fatty acid end of the surfactin are as follows:
 (1) iso-C13>3%; 
 (2) C13>0.65%; 
 (3) iso-C14>17%; 
 (4) C14<41%; and 
 (5) iso-C15<11%. 
 
     
     
         20 . The method as recited in  claim 19 , wherein the distribution of the fatty acids at the fatty acid end of the surfactin are as follows:
 (1) iso-C13>10%;   (2) C13>25%;   (3) iso-C14>35%;   (4) C14<25%; and   (5) iso-C15<3%.   
     
     
         21 . The method as recited in  claim 20 , wherein the distribution of the fatty acids at the fatty acid end of the surfactin are as follows: (1) iso-C 13=11%; (2) C13=26%; (3) iso-C 14=37%; (4) C14=24%; and (5) iso-C15=2%. 
     
     
         22 . The method as recited in  claim 18 , wherein the molecular weight of the surfactin is 1022 or 1036 Da. 
     
     
         23 . The method as recited in  claim 18 , wherein the surfactin comprises its isomers. 
     
     
         24 . The method as recited in  claim 18 , wherein the emulsifying composition further comprises at least one of the following ingredients: glycerol fatty acid esters, sorbitan fatty acid esters, sucrose fatty acid esters, propylene glycol fatty acid esters, and lecithin. 
     
     
         25 . The method as recited in  claim 18 , wherein the emulsifying composition is for increasing foaming power. 
     
     
         26 . The method as recited in  claim 18 , wherein the emulsifying composition is for increasing emulsification. 
     
     
         27 . The method as recited in  claim 18 , wherein the emulsifying composition is for increasing the sense of smooth.