Method for Detection of Target Nucleic Acid

Abstract

An object of the disclosure of the present specification is to provide a method for detection of a target nucleic acid which allows construction of an effective detection system of a target nucleic acid. For this purpose, in the disclosure of the present specification, a first primer comprising an identification sequence complementary to a target sequence in a target nucleic acid and a tag addition sequence, and a second primer having a label are prepared. The first primer and the second primer are used for the target nucleic acid in a sample to amplify a chimeric DNA having a tag sequence and the label. The chimeric DNA is hybridized with a detection probe on a solid phase to obtain signal intensity information based on the label, and the target nucleic acid is detected based on the signal intensity information.

1 . A set of primers for use in a method of detection of a target nucleic acid, wherein the set of priers comprises:
 a first primer having an identification sequence complementary to a target sequence in a target nucleic acid and a tag addition sequence complementary to a tag sequence, wherein the tag sequence is complementary to a detection probe correlated to the target nucleic acid; and   a second primer having a partial sequence that has the same sequence as a partial sequence adjacent to the target sequence, wherein the set of primers does not include a universal primer.   
     
     
         2 . The set of primers of  claim 1 ,
 wherein the set of primers consists of a first primer having an identification sequence complementary to a target sequence in a target nucleic acid and a tag addition sequence complementary to a tag sequence, and   wherein the tag sequence is complementary to a detection probe correlated to the target nucleic acid, and a second primer having a partial sequence that has the same sequence as a partial sequence adjacent to the target sequence.   
     
     
         3 . A kit for carrying out the detection of a target nucleic acid, said kit comprising;
 a set of detection probes bound to a solid support, wherein the detection probes have different base sequences;   a set of primers a first primer having an identification sequence complementary to a target sequence in a target nucleic acid and a tag addition sequence complementary to a tag sequence, wherein the tag sequence is complementary to a detection probe correlated to the target nucleic acid, and   a second primer having a partial sequence that has the same sequence as a partial sequence adjacent to the target sequence, wherein the set of primers does not include a universal primer.   
     
     
         4 . A kit for the detection of a target nucleic acid, comprising;
 an array comprising a set of detection probes respectively having different base sequences, said probes bound to a solid support; and   a set of primers which does not include a universal primer,   wherein the set of primers consists of a first primer having an identification sequence complementary to a target sequence in a target nucleic acid and a tag addition sequence complementary to a tag sequence, and   wherein the tag sequence is complementary to a detection probe correlated to the target nucleic acid, and a second primer having a partial sequence that has the same sequence as a partial sequence adjacent to the target sequence and the label a detection probe hybridizable to the tag sequence having been correlated to the target nucleic acid.