Methods and articles of manufacture for the treatment of skin

Abstract

Embodiments of the present invention are directed to methods and articles of manufacture to treat skin to improve and/or increase hydration, pliability, and thickness for improved texture, feel and appearance. Embodiments feature applying an effective amount of an acetylation agent to natural dermal collagen under reaction conditions to react the natural dermal collagen with the acetylation agent to form a modified collagen. The modified collagen has a higher net charge and higher net charge density than natural dermal collagen. The modified collagen improves or increases one or more skin characteristics consisting of hydration, pliability and thickness.

1 . A method of treating skin comprising the steps of:
 applying an effective amount of an acetylation agent to natural dermal collagen under reaction conditions to react said natural dermal collagen with said acetylation agent to form a modified collagen, said modified collagen having higher net charge and higher net charge density than natural dermal collagen, said modified collagen for increasing one or more skin characteristics consisting of hydration, pliability and thickness.   
     
     
         2 . The method of  claim 1  wherein said skin to be treated has an area and further comprising the step of treating the area to allow penetration of the acetylation agent through the stratum corneum and epithelium of the skin. 
     
     
         3 . The method of  claim 2  wherein said step of treating comprising one or more skin preparation treatments selected from the group consisting of dermal abrasion, micro-needle puncture, abrasive washes and air jet injection. 
     
     
         4 . The method of  claim 1  wherein said effective amount of an acetylation agent is in a deliver system selected from the group consisting of foams, ointments, pastes, oils, creams, solutions, tinctures, gels, suspensions, powders, aerosols and emulsions. 
     
     
         5 . The method of  claim 4  wherein said deliver system has an aqueous base and said acetylation agent is held in a buffered solution at a pH of 8.0 to 10.0. 
     
     
         6 . The method of  claim 4  wherein said deliver system has an oil base and said acetylation agent is held as a suspension. 
     
     
         7 . The method of  claim 5  wherein said acetylation agent has a concentration in said aqueous base and said concentration is 1 mg to 100 mg/ml. 
     
     
         8 . The method of  claim 4  wherein said deliver system has an alcohol base and said acetylation agent has a concentration of 1 to 100 mg/ml. 
     
     
         9 . The method of  claim 6  wherein said acetylation agent has a concentration of 1 to 100 mg/ml. 
     
     
         10 . The method of  claim 4  wherein said delivery system has a penetration enhancer. 
     
     
         11 . The method of  claim 10  wherein said penetration enhancer is ethylenediaminetetraacetic acid and salts thereof. 
     
     
         12 . The method of  claim 11  wherein said ethylenediaminetetraacetic acid is present in a concentration of 0.022 M to 0.040 M. 
     
     
         13 . The method of  claim 1  further comprises one or more post application treatments comprising the application of washes. 
     
     
         14 . The method of  claim 13  wherein said wash is selected from the group of solutions consisting of aqueous solutions, phosphate buffered solutions, saline solutions and surfactant solutions and combinations thereof. 
     
     
         15 . The method of  claim 1  wherein to said acetylation agent is selected from the group consisting of maleic anhydride, succinic anhydride, glutaric anhydride, citractonic anhydride, methyl succinic anhydride, itaconic anhydride, methyl glutaric anhydride, dimethyl glutaric anhydride, phthalic anhydride, oxalyl chloride, malonyl chloride, chlorosulfonylacetyl chloride, chlorosulfonylbenzoic acid, 4-chloro-3-(chlorosulfonyl)-5-nitroebnzoic acid, 3-(chlorosulfonyl)-P-anisic acid, 3-sulfobenzoic acid, 3,5-dicarboxybenzenesulfonyl chloride, acetic anhydride, chloroacetic anhydride, propionic anhydride, butyric anhydride, isobutyric anhydride, isovaleric anhydride, hexanoic anhydride, acetyl chloride, propionyl chloride, dichloropropionyl chloride, butyryl chloride, isobutyryl chloride, valeryl chloride, ethane sulfonyl chloride, methane sulfonyl chloride, 1-butane sulfonyl chloride, 4,6-diamino-2-methylthiopyrimidine-5-sulfonic acid, and mixtures and combinations thereof. 
     
     
         16 . The method of  claim 1  wherein said acetylation agent is glutaric anhydride or a methyl derivative thereof applied to the natural dermal collagen in an aqueous base at a pH of 8.0-10. 
     
     
         17 . The method of  claim 1  wherein said acetylation agent is glutaric anhydride or a methyl derivative thereof held in an oil base. 
     
     
         18 . As an article of manufacture, a delivery system for the treatment of skin comprising an effective amount of an acetylation agent to apply to natural dermal collagen under reaction conditions to react said natural dermal collagen with said acetylation agent to form a modified collagen, said modified collagen having higher net charge and higher net charge density than natural dermal collagen, said modified collagen for increasing one or more skin characteristics consisting of hydration, pliability and thickness. 
     
     
         19 . The article of manufacture of  claim 18  wherein said skin to be treated has an area and further comprising penetration means for treating the area to allow penetration of the acetylation agent through the stratum corneum and epithelium of the skin. 
     
     
         20 . The article of manufacture of  claim 20  wherein said penetration means comprises one or more skin preparation treatments selected from the group consisting of dermal abrasion elements, micro-needles, needles for injection, hollow micro-needles, abrasive washes and air jet injection. 
     
     
         21 . The article of manufacture of  claim 18  wherein said deliver system is selected from the group consisting of transdermal patches, foams, ointments, pastes, oils, creams, solutions, tinctures, gels, suspensions, powders, aerosols and emulsions. 
     
     
         22 . The article of manufacture of  claim 21  wherein said deliver system has an aqueous base and said acetylation agent is held in a buffered solution at a pH of 8.0 to 10.0. 
     
     
         23 . The article of manufacture of  claim 21  wherein said deliver system has an oil base and said acetylation agent is held as a suspension. 
     
     
         24 . The article of manufacture of  claim 22  wherein said acetylation agent has a concentration in said aqueous base and said concentration is 1 mg to 100 mg/ml. 
     
     
         25 . The article of manufacture of  claim 21  wherein said deliver system has an alcohol base and said acetylation agent has a concentration of 1 to 100 mg/ml. 
     
     
         26 . The article of manufacture of  claim 23  wherein said acetylation agent has a concentration of 1 to 100 mg/ml. 
     
     
         27 . The article of manufacture of  claim 21  wherein said delivery system has a penetration enhancer. 
     
     
         28 . The article of manufacture of  claim 27  wherein said penetration enhancer is ethylenediaminetetraacetic acid and salts thereof. 
     
     
         29 . The article of manufacture of  claim 28  wherein said ethylenediaminetetraacetic acid is present in a concentration of 0.022 M to 0.040 M. 
     
     
         30 . The article of manufacture of  claim 18  further comprising at least one post application treatments. 
     
     
         31 . The article of manufacture of  claim 30  wherein said at least one post application treatments is a wash. 
     
     
         32 . The article of manufacture of  claim 31  wherein said wash is selected from the group of solutions consisting of aqueous solutions, phosphate buffered solutions, saline solutions and surfactant solutions and combinations thereof. 
     
     
         33 . The article of manufacture of  claim 18  wherein said acetylation agent is selected from the group consisting of maleic anhydride, succinic anhydride, glutaric anhydride, citractonic anhydride, methyl succinic anhydride, itaconic anhydride, methyl glutaric anhydride, dimethyl glutaric anhydride, phthalic anhydride, oxalyl chloride, malonyl chloride, chlorosulfonylacetyl chloride, chloro sulfonylbenzoic acid, 4-chloro-3-(chlorosulfonyl)-5-nitroebnzoic acid, 3-(chlorosulfonyl)-P-anisic acid, 3-sulfobenzoic acid, 3,5-dicarboxybenzenesulfonyl chloride, acetic anhydride, chloroacetic anhydride, propionic anhydride, butyric anhydride, isobutyric anhydride, isovaleric anhydride, hexanoic anhydride, acetyl chloride, propionyl chloride, dichloropropionyl chloride, butyryl chloride, isobutyryl chloride, valeryl chloride, ethane sulfonyl chloride, methane sulfonyl chloride, 1-butane sulfonyl chloride, 4,6-diamino-2-methylthiopyrimidine-5-sulfonic acid, and mixtures and combinations thereof. 
     
     
         34 . The article of manufacture of  claim 33  wherein said acetylation agent is glutaric anhydride or a methyl derivative thereof applied to the natural dermal collagen in an aqueous base at a pH of 8.0-10. 
     
     
         35 . The article of manufacture of  claim 33  wherein said acetylation agent is glutaric anhydride or a methyl derivative thereof held in an oil base. 
     
     
         36 . The article of manufacture of  claim 18  wherein said acetylation agent is combined with an aqueous base immediately prior to application. 
     
     
         37 . The article of manufacture of  claim 36  wherein said acetylation agent is held in a first containment vessel and said aqueous base is held in a second containment vessel and combined in a mixing unit immediately prior to administration to dermal collagen. 
     
     
         38 . The article of manufacture of  claim 37  wherein said acetylation agent is applied by jet spray. 
     
     
         39 . The article of manufacture of  claim 37  wherein said first containment vessel and said second containment vessel are foil packs. 
     
     
         40 . The article of manufacture of  claim 39  further comprising at least one pad for administration of said acetylation agent.