Novel genes and proteins of brachyspira hyodysenteriae and uses thereof

Abstract

Novel polynucleotide and amino acids of Brachyspira hyodysenteriae are described. These sequences are useful for diagnosis of B. hyodysenteriae disease in animals and as a therapeutic treatment or prophylactic treatment of B. hyodysenteriae disease in animals. These sequences may also be useful for diagnostic and therapeutic and/or prophylactic treatment of diseases in animals caused by other Brachyspira species, including B. intermedia, B. suantatina, B. alvinipulli, B. aalborgi, B. innocens, B. murdochii , and B. pilosicoli.

1 . An isolated polypeptide comprising the full length of an amino acid sequence selected from the group consisting of SEQ ID NO: 22, 32, and 64 with a heterologous polypeptide that permits the detection, isolation, solubilization, or stabilization of the isolated polypeptide. 
     
     
         2 - 5 . (canceled) 
     
     
         6 . An immunogenic composition comprising the polypeptide of  claim 1 . 
     
     
         7 - 30 . (canceled) 
     
     
         31 . A method of generating an immune response to  Brachyspira  infection in an animal comprising administering to said animal the polypeptide of  claim 1 . 
     
     
         32 - 44 . (canceled) 
     
     
         45 . An isolated polypeptide comprising a sequence that is at least 70% homologous to a polypeptide with an amino acid sequence selected from the group consisting of SEQ ID NO: 22, 32, and 64 and a heterologous polypeptide that permits the detection, isolation, solubilization, or stabilization of the isolated polypeptide. 
     
     
         46 . An isolated polypeptide comprising a sequence that is at least 80% homologous to a polypeptide with an amino acid sequence selected from the group consisting of SEQ ID NO: 22, 32, and 64 and a heterologous polypeptide that permits the detection, isolation, solubilization, or stabilization of the isolated polypeptide. 
     
     
         47 . An isolated polypeptide comprising a sequence that is at least 90% homologous to a polypeptide with an amino acid sequence selected from the group consisting of SEQ ID NO: 22, 32, and 64 and a heterologous polypeptide that permits the detection, isolation, solubilization, or stabilization of the isolated polypeptide. 
     
     
         48 . An immunogenic composition comprising the polypeptide of  claim 45 . 
     
     
         49 . A method of generating an immune response to  Brachyspira  infection in an animal comprising administering to said animal the polypeptide of  claim 45 . 
     
     
         50 . An immunogenic composition comprising the polypeptide of  claim 46 . 
     
     
         51 . A method of generating an immune response to  Brachyspira  infection in an animal comprising administering to said animal the polypeptide of  claim 46 . 
     
     
         52 . An immunogenic composition comprising the polypeptide of  claim 47 . 
     
     
         53 . A method of generating an immune response to  Brachyspira  infection in an animal comprising administering to said animal the polypeptide of  claim 47 .