Combination Treatment of Cardiovascular Disease

Abstract

Disclosed are methods, compositions of matter and cells for treatment of cardiovascular disease through concurrent inhibition of oxidative stress while administration of a cell therapy. The invention also concerts the modulation of oxidative stress for preferential induction of differentiation while concurrently inhibiting inflammatory processes that decrease efficacy of cellular therapy.

What is claimed is: 
     
         1 . A method of amerliorating the effects of myocardial infarction comprising: a) identifying a subject who has suffered from a myocardial infarction b) harvesting mesenchymal stem cells; c) intravenously injecting said mesenchymal stem cells into said subject; and d) administering ascorbic acid or a derivative thereof intravenously to said subject to ameliorate the effects of myocardial infarction. 
     
     
         2 . The method of  claim 1 , wherein said ascorbic acid is administered intravenously in an amount of 15-700 g/week. 
     
     
         3 . The method of  claim 2 , wherein said ascorbic acid is administered intravenously in an amount of 25 g/day. 
     
     
         4 . The method of  claim 1 , wherein said ascorbic acid is intravenously administered to said subject prior to the administration of said mesenchymal stem cells. 
     
     
         5 . The method of  claim 1 , further comprising measuring oxidative stress in said subject prior to administration of said mesenchymal stem cells and wherein the ascorbic acid is intravenously administrated at a concentration and frequency based upon said measurement of oxidative stress. 
     
     
         6 . The method of  claim 1 , wherein the subject is tested after being administered the ascorbic acid and mesenchymal cells and there is an improvement in ejection fraction and clinical heart failure score. 
     
     
         7 . The method of  claim 1 , wherein the subject is tested after being administered the ascorbic acid and mesenchymal stem cells and there is an improvement in ejection fraction. 
     
     
         8 . The method of  claim 1 , wherein the subject is tested after being administered the ascorbic acid and mesenchymal stem cells and there is an improvement in clinical heart failure score.