Sealants, manufacturing thereof and applications thereof

Abstract

Method comprising: applying uncured pre-gel (UP to surface; contacting UP with cross-linking agents; allowing applied UP to cure, increasing adhere to first surface, source of agents; solid support comprising agent, or insoluble agent salt in UP, wherein trigger compound is added to UP, or spraying/coating agent, with solid support added.

We claim: 
     
         1 . A multi component adhesive comprising:
 a) an uncured pre-gel comprising at least one water miscible cross-linkable polymer selected from the group consisting of naturally existing form of a carbohydrate, a synthetically prepared form of carbohydrate and a salt of a polysaccharide; and   b) a biocompatible solid support comprising at least one cross linking agent or water-soluble salt thereof,   wherein the uncured pre-gel applied to a first surface is capable of curing and increasingly adhering to the surface after adding the solid support to the uncured pre-gel.   
     
     
         2 . The multi component adhesive of  claim 1 , wherein the solid support comprises the salt of the cross-linking agent, the uncured pre-gel further comprising an aqueous liquid capable of dissolving the salt of the cross linking agent after adding the composition comprising the trigger compound to the uncured pre-gel. 
     
     
         3 . The multi component adhesive of  claim 1 , wherein said solid support comprises the salt of the cross-linking agent and both the pre-gel and the solid support are dry, wherein curing and adhering to the first surface occurs upon adding an aqueous liquid capable of dissolving the salt to the solid support and/or uncured pre-gel, after adding the solid support to the uncured pre-gel. 
     
     
         4 . The multi component adhesive of  claim 1 , further comprising a biocompatible solid support. 
     
     
         5 . The multi component adhesive of  claim 4 , wherein the biocompatible solid support is bioabsorbable. 
     
     
         6 . The multi component adhesive of  claim 5 , wherein the solid support is one or more materials selected from the group consisting of: poly(α-caprolactone) (PCL), poly(glycolide) (PGA), poly(lactide) (PLA), and poly(glycolide-co-lactide) (PLGA), and oxidized regenerated cellulose, and hydrophilic water-soluble polymer, selected from one or more of the group comprising: alginate, polyethylene glycol, polyvinyl alcohol, dextran, and pectin. 
     
     
         7 . The multi component adhesive of  claim 5 , wherein the solid support comprises a mesh. 
     
     
         8 . The multi component adhesive of  claim 7 , wherein the mesh comprises a fibrous structure. 
     
     
         9 . The multi component adhesive of  claim 1 , wherein said pre-gel further comprises non-soluble suspended solids. 
     
     
         10 . The multi component adhesive as claimed in  claim 9 , wherein said suspended solids are in the form of particles comprising fibers. 
     
     
         11 . The multi component adhesive as claimed in  claim 1 , wherein said at least one cross linking agent comprises multivalent charged materials made from dissolution of one or more of a group comprising polyelectrolytes, organic salts, and inorganic salts. 
     
     
         12 . The multi component adhesive as claimed in  claim 1 , further comprising one or more therapeutic materials selected from one or more of a group comprising drugs, therapeutic proteins, growth factors, and hormones. 
     
     
         13 . The multi component adhesive of  claim 1 , wherein the cured pre-gel is capable of being an adhesion barrier to a second surface, wherein said second surface is selected from a group of tissue surface, synthetic graft surface, and organ surface. 
     
     
         14 . A method of treating a subject comprising:
 a) providing an uncured pre-gel comprising at least one water miscible cross-linkable polymer selected from the group comprising naturally existing form of a carbohydrate, a synthetically prepared form of carbohydrate and a salt of a polysaccharide;   b) providing a biocompatible solid support comprising at least one cross linking agent or water-soluble salt thereof;   c) applying the uncured pre-gel to a first surface on or within the subject; subsequently contacting the uncured pre-gel with one or more cross linking agents; and   d) subsequently allowing the applied uncured pre-gel to cure and increasingly adhere to the first surface.   
     
     
         15 . The method of  claim 14 , further comprising adding an aqueous liquid, capable of dissolving the salt to the uncured pre-gel and/or solid support. 
     
     
         16 . The method of  claim 15 , further comprising one or more of the actions selected from the group consisting of: embedding a biocompatible solid support in the uncured pre-gel; placing the biocompatible solid support on the uncured pre-gel, and soaking said biocompatible solid support with said uncured pre-gel. 
     
     
         17 . The method of  claim 14 , wherein the biocompatible solid support is bioabsorbable. 
     
     
         18 . The method of  claim 14 , wherein said first surface is selected from a group comprising tissue surface, synthetic graft surface, and organ surface. 
     
     
         19 . The method of  claim 14 , wherein said application of adhesive comprises sealing or closing an opening in the first surface. 
     
     
         20 . The method of  claim 14 , further comprising sealing suture lines of end-to-side anastomosis of grafts.