Medium for culturing stem cells

Abstract

Culture media, which contain albumin carrying a reduced amount of fatty acid, are useful for culturing stem cells.

1 . A medium for culturing stem cells, comprising an albumin which comprises a reduced amount of fatty acid. 
     
     
         2 . The medium according to  claim 1 , wherein said albumin which comprises a reduced amount of fatty acid is obtained by subjecting albumin to a fatty acid removal treatment. 
     
     
         3 . The medium according to  claim 2 , wherein said fatty acid removal treatment comprises treating albumin with activated carbon. 
     
     
         4 . The medium according to  claim 1 , wherein said albumin comprises fatty acid in an amount of 0.1 mg/g to 0.65 mg/g, based on the total amount of said albumin. 
     
     
         5 . A medium for culturing stem cells, which comprises fatty acid in an amount of 0.1 mg to 0.65 mg per 1 g albumin in said medium. 
     
     
         6 . The medium according to  claim 1 , wherein said fatty acid is a long chain fatty acid. 
     
     
         7 . The medium according to  claim 1 , wherein said fatty acid is at least one member selected from the group consisting of oleic acid, palmitic acid, stearic acid, linoleic acid, linolenic acid, and arachidonic acid. 
     
     
         8 . The medium according to  claim 1 , wherein said albumin is a human serum-derived albumin. 
     
     
         9 . A method of culturing stem cells, comprising cultivating stem cells in a medium according to  claim 1 . 
     
     
         10 . The method according to  claim 9 , wherein said stem cells are pluripotent stem cells. 
     
     
         11 . The method according to  claim 10 , wherein said pluripotent stem cells are embryonic stem cells (ES cells) or induced pluripotent stem cells (iPS cells). 
     
     
         12 . A method of selecting an albumin suitable for addition to a medium, comprising:
 measuring the amount of fatty acid comprised in an albumin; and   selecting an albumin which comprises a reduced amount of fatty acid.   
     
     
         13 . A method of producing a medium for culturing stem cells, comprising:
 preparing an albumin comprising a reduced amount of fatty acid by a fatty acid removal treatment; and   adding said albumin comprising a reduced amount of fatty acid to a medium.   
     
     
         14 . The method according to  claim 13 , wherein said fatty acid removal treatment comprises treating albumin with activated carbon. 
     
     
         15 . The method according to  claim 14 , wherein said albumin is treated with 30 to 60 wt % of activated carbon, based on the weight of said albumin. 
     
     
         16 . The method according to  claim 14 , wherein said treating albumin with activated carbon is conducted at a pH of 6.7 to 7.3. 
     
     
         17 . The method according to  claim 14 , wherein said treating albumin with activated carbon is conducted at a pH of 3.7 to 4.3. 
     
     
         18 . The method according to  claim 13 , wherein said albumin comprises fatty acid in an amount of 0.1 mg/g to 0.65 mg/g, based on the amount of albumin. 
     
     
         19 . The method according to  claim 13 , wherein said fatty acid is a long chain fatty acid. 
     
     
         20 . The method according to  claim 13 , wherein said fatty acid is at least one member selected from the group consisting of oleic acid, palmitic acid, stearic acid, linoleic acid, linolenic acid, and arachidonic acid. 
     
     
         21 . The method according to  claim 13 , wherein said albumin is human serum-derived albumin. 
     
     
         22 . The medium according to  claim 2 , wherein said albumin comprises fatty acid in an amount of 0.1 mg/g to 0.65 mg/g, based on the total amount of said albumin. 
     
     
         23 . The medium according to  claim 3 , wherein said albumin comprises fatty acid in an amount of 0.1 mg/g to 0.65 mg/g, based on the total amount of said albumin. 
     
     
         24 . A cell culture, comprising stem cells in a culture medium according to  claim 1 . 
     
     
         25 . The cell culture according to  claim 24 , wherein said stem cells are pluripotent stem cells. 
     
     
         26 . The cell culture according to  claim 25 , wherein said pluripotent stem cells are embryonic stem cells (ES cells) or induced pluripotent stem cells (iPS cells).