Silk fibroin hydrogels and uses thereof

Abstract

The present specification provides for methods for purifying fibroins, purified fibroins, methods of conjugating biological and synthetic molecules to fibroins, fibroins conjugated to such molecules, methods of making fibroin hydrogels, fibroin hydrogels and fibroin hydrogel formulations useful for a variety of medical uses, including, without limitation uses as bulking agents, tissue space fillers, templates for tissue reconstruction or regeneration, cell culture scaffolds for tissue engineering and for disease models, surface coating to improve medical device function, or drug delivery devices.

1 . A method of breast reconstruction or augmentation, the method comprising the steps of
 implanting a silicone or saline filled breast implant into an individual; and   implanting a hydrogel scaffold at the site of implantation, the hydrogel scaffold comprising a substantially sericin-depleted silk fibroin and adipose cells autologous to the individual, the hydrogel scaffold formed by:
 extracting sericin from a silk fibroin thereby forming the substantially sericin-depleted silk fibroin, 
 digesting a mixture including the substantially sericin-depleted silk fibroin, thereby forming a digested mixture, wherein the digested mixture comprises depolymerized silk fibroin, 
 gelling the depolymerized silk fibroin in an ethanol solution thereby forming a silk fibroin hydrogel, and 
 seeding the silk fibroin hydrogel with adipose cells autologous to the individual.