US2016097029A1PendingUtilityA1

Engineered cyanobacteria with enhanced salt tolerance

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Assignee: MORGAN STATE UNIVERSITYPriority: Oct 6, 2014Filed: Oct 2, 2015Published: Apr 7, 2016
Est. expiryOct 6, 2034(~8.2 yrs left)· nominal 20-yr term from priority
Inventors:Viji SittherBehnam Tabatabai
C07K 14/195C12P 7/6463C12N 1/20C12N 15/74C12P 7/64C12R 2001/01C12N 1/205
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Claims

Abstract

The present invention relates to a recombinant cyanobacterium with enhanced halotolerance and compositions thereof, methods of producing the recombinant cyanobacterium, and methods of using the same for biofuel production. The invention also relates to transformed F. diplosiphon strains with enhanced salt tolerance.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 . A recombinant cyanobacterium comprising at least one polynucleotide encoding a halotolerance gene. 
     
     
         2 . The recombinant cyanobacterium according to  claim 1 , wherein the recombinant cyanobacterium is  Fremyella diplosiphon.    
     
     
         3 . The recombinant cyanobacterium according to  claim 1 , wherein the halotolerance gene is selected from a group consisting of ApNhaP, BetT, Mdh, ApNapA, ApGMST, ApDMT, and HlyB. 
     
     
         4 . The recombinant cyanobacterium according to  claim 1 , wherein the halotolerance gene is a homologue isolated from  Aphanothece halophytica.    
     
     
         5 . The recombinant cyanobacterium according to  claim 1 , wherein the recombinant cyanobacterium has a higher salt tolerance as compared to wild-type cyanobacterium. 
     
     
         6 . The recombinant cyanobacterium according to  claim 1 , wherein the at least one polynucleotide is present in one or more plasmids. 
     
     
         7 . A method for producing biofuel comprising:
 growing a recombinant cyanobacterium comprising at least one polynucleotide encoding a halotolerance gene under conditions suitable for production of a lipid for biofuel production;   and isolating the lipid.   
     
     
         8 . The method according to  claim 7 , wherein the recombinant cyanobacterium is  Fremyella diplosiphon.    
     
     
         9 . The method according to  claim 7 , wherein the halotolerance gene is selected from a group consisting of ApNhaP, BetT, Mdh, ApNapA, ApGMST, ApDMT, and HlyB. 
     
     
         10 . The method according to  claim 7 , wherein the halotolerance gene is a homologue isolated from  Aphanothece halophytica.    
     
     
         11 . The method according to  claim 7 , wherein the recombinant cyanobacterium has a higher salt tolerance as compared to wild-type cyanobacterium. 
     
     
         12 . The method according to  claim 7 , wherein at least one polynucleotide encoding a halotolerance gene is incorporated in one or more plasmids and integrated into genome of the cyanobacterium. 
     
     
         13 . A method for producing a halotolerant recombinant  Fremyella diplosiphon  comprising:
 introducing at least one polynucleotide encoding a halotolerance gene into  F. diplosiphon.      
     
     
         14 . The method for producing a halotolerant recombinant  F. diplosiphon  according to  claim 13 , wherein the halotolerance gene is selected from a group consisting of ApNhaP, ApGMST, ApDMT, Mdh, and HlyB. 
     
     
         15 . The method for producing a halotolerant recombinant  F. diplosiphon  according to  claim 13 , wherein the halotolerance gene is isolated from  Aphanothece halophytica.    
     
     
         16 . The method for producing a halotolerant recombinant  F. diplosiphon  according to  claim 13 , wherein the recombinant  F. diplosiphon  has a higher salt tolerance as compared to wild-type  F. diplosiphon.    
     
     
         17 . The method for producing a halotolerant recombinant  F. diplosiphon  according to  claim 13 , further comprising a step of incorporating at least one polynucleotide encoding a halotolerance gene in one or more plasmids and transforming one or more plasmids into a genome of the  F. diplosiphon.    
     
     
         18 . A plasmid comprising at least one nucleic acid encoding a halotolerance gene. 
     
     
         19 . The plasmid according to  claim 19 , wherein the halotolerance gene is selected from the group consisting of ApNhaP, ApGMST, ApDMT, Mdh, and HlyB. 
     
     
         20 . The plasmid according to  claim 19 , wherein the halotolerance gene is isolated from  Aphanothece halophytica.    
     
     
         21 . A recombinant strain of  F. diplosiphon  having the characteristics of that deposited in KR912179, or a mutant thereof. 
     
     
         22 . A recombinant strain of  F. diplosiphon  having the characteristics of that deposited in KP036997.1, or a mutant thereof. 
     
     
         23 . A composition comprising the recombinant strain of  claim 1 ,  claim 21 , or  claim 22 .

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