Method for determining p1/p2 blood type and detection kit thereof

Abstract

The present invention provides a method for determining P 1 /P 2 blood type, including steps of providing a biological sample of a subject, detecting a genotype for single nucleotide polymorphism rs2143918 or rs5751348 in A4GALT gene of the biological sample and determining a phenotype of the subject based on the genotype. Further, the present invention also provides a kit for determining P 1 /P 2 blood type, including a primer pair for detecting a genotype for single nucleotide polymorphisms rs2143918 or rs5751348 in A4GALT gene of a nucleic acid sample of a subject.

What is claimed is: 
     
         1 . A method for determining P 1 /P 2  blood type, comprising steps of:
 providing a biological sample of a subject;   detecting a genotype for a single nucleotide polymorphism at rs2143918 or rs5751348 in A4GALT gene of the biological sample; and   determining a phenotype of the subject based on the genotype.   
     
     
         2 . The method of  claim 1 , wherein the genotype of the single nucleotide polymorphism at rs2143918 is selected from the group consisting of T/T, T/G and GIG. 
     
     
         3 . The method of  claim 1 , wherein T/T or T/G genotype of the single nucleotide polymorphism at rs2143918 represents P 1  phenotype in the subject. 
     
     
         4 . The method of  claim 1 , wherein G/G genotype of the single nucleotide polymorphism at rs2143918 represents P 2  phenotype in the subject. 
     
     
         5 . The method of  claim 1 , wherein the genotype of the single nucleotide polymorphism at rs5751348 is selected from the group consisting of G/G, G/T and T/T. 
     
     
         6 . The method of  claim 1 , wherein G/G or G/T genotype of the single nucleotide polymorphism at rs5751348 represents P 1  phenotype in the subject. 
     
     
         7 . The method of  claim 1 , wherein T/T genotype of the single nucleotide polymorphism at rs5751348 represents P 2  phenotype in the subject. 
     
     
         8 . The method of  claim 1 , wherein the biological sample is blood or saliva. 
     
     
         9 . The method of  claim 1 , wherein a nucleic acid in the biological sample is determined by polymerase chain reaction (PCR). 
     
     
         10 . The method of  claim 9 , wherein a primer pair of SEQ ID Nos: 1 and 2 are used in the PCR. 
     
     
         11 . The method of  claim 9 , wherein a primer pair of SEQ ID Nos: 3 and 4 are used in the PCR. 
     
     
         12 . A detection kit for determining P 1 /P 2  blood type, comprising:
 a primer pair for determining a genotype of a single nucleotide polymorphism at rs2143918 or rs5751348 of A4GALT gene in a nucleic acid sample of a subject.   
     
     
         13 . The detection kit of  claim 12 , wherein the primer pair has sequences of SEQ ID Nos:
 1 and 2.   
     
     
         14 . The detection kit of  claim 12 , wherein the primer pair has sequences of SEQ ID Nos:
 3 and 4.   
     
     
         15 . The detection kit of  claim 12 , wherein the genotype of the single nucleotide polymorphism at rs2143918 is selected from the group consisting of T/T, T/G and G/G. 
     
     
         16 . The detection kit of  claim 15 , wherein T/T or T/G genotype of the single nucleotide polymorphism at rs2143918 represents P 1  phenotype in the subject. 
     
     
         17 . The detection kit of  claim 15 , wherein G/G genotype of the single nucleotide polymorphism at rs2143918 represents P 2  phenotype in the subject. 
     
     
         18 . The detection kit of  claim 12 , wherein the genotype of the single nucleotide polymorphism at rs5751348 is selected from the group consisting of G/G, G/T and T/T. 
     
     
         19 . The detection kit of  claim 18 , wherein G/G or G/T genotype of the single nucleotide polymorphism at rs5751348 represents P 1  phenotype in the subject. 
     
     
         20 . The detection kit of  claim 18 , wherein T/T genotype of the single nucleotide polymorphism at rs5751348 represents P 2  phenotype in the subject.