Multiple Inducible Gene Regulation System

Abstract

The present invention relates to the field of biotechnology or genetic engineering. More specifically, the present invention relates to a multiple inducible gene regulation system that functions within cells to simultaneously control the quantitative expression of multiple genes.

1 - 20 . (canceled) 
     
     
         21 . A vector encoding a multiple inducible gene modulation, the vector comprising:
 A. 1) a) a first polynucleotide encoding a first ligand-inducible transcription factor, wherein the first polynucleotide comprises SEQ ID NO: 1 and SEQ ID NO: 2,
 b) a first promoter operably linked to the first polynucleotide, wherein the first promoter comprises SEQ ID NO: 3; 
 2) a) a second polynucleotide encoding a second ligand-inducible transcription factor, wherein the second polynucleotide comprises SEQ ID NO: 4 and SEQ ID NO: 5, 
 b) a second promoter operably linked to the second polynucleotide, wherein the second promoter comprises SEQ ID NO: 3; 
 3) a) a third polynucleotide encoding a co-activation domain, wherein the third polynucleotide comprises SEQ ID NO: 6 and SEQ ID NO: 7, 
 b) a third promoter operably linked to the third polynucleotide, wherein the third promoter comprises SEQ ID NO: 3; 
 1) a first gene expression cassette comprising: 
 a) a first response element recognized by the first ligand-inducible transcription factor, wherein the first response element comprises SEQ ID NO: 8, 
 b) a fourth promoter that is activated by a transactivation domain from the co-activation domain, wherein the fourth promoter comprises SEQ ID NO: 9, and 
 c) a first gene whose expression is to be modulated; and 
 2) a second gene expression cassette comprising: 
 a) a second response element recognized by the second ligand-inducible transcription factor, wherein the second response element comprises SEQ ID NO: 11, 
 b) a fifth promoter that is activated by a transactivation domain from the co-activation domain, wherein the fifth promoter comprises SEQ ID NO: 12, and 
 c) a second gene whose expression is to be modulated; or 
   B. 1) a) a first polynucleotide encoding a first ligand-inducible transcription factor, wherein the first polynucleotide comprises SEQ ID NO: 14 and SEQ ID NO: 2,
 b) a first promoter operably linked to the first polynucleotide, wherein the first promoter comprises SEQ ID NO: 3; 
 4) a) a second polynucleotide encoding a second ligand-inducible transcription factor, wherein the second polynucleotide comprises SEQ ID NO: 15 and SEQ ID NO: 2, 
 b) a second promoter operably linked to the second polynucleotide, wherein the second promoter comprises SEQ ID NO: 3; 
 5) a) a third polynucleotide encoding a co-activation domain, wherein the third polynucleotide comprises SEQ ID NO: 16 and SEQ ID NO: 7, 
 b) a third promoter operably linked to the third polynucleotide, wherein the third promoter comprises SEQ ID NO: 3; and 
 1) a gene expression cassette comprising: 
 a) a first response element recognized by the first and the second ligand-inducible transcription factor, wherein the first response element comprises SEQ ID NO: 8, 
 b) a fourth promoter that is activated by a transactivation domain from the co-activation domain, wherein the fourth promoter comprises SEQ ID NO: 12, and 
 c) a gene whose expression is to be modulated. 
   
     
     
         22 . A virus comprising the vector of  claim 21 . 
     
     
         23 . An host cell comprising the vector of  claim 21 . 
     
     
         24 . A transgenic organism comprising one or more cells of  claim 23 . 
     
     
         25 . An isolated host cell comprising the vector of  claim 21 . 
     
     
         26 . The isolated host cell of  claim 25 , wherein the host cell is a bacterial cell, a fungal cell, a yeast cell, a plant cell, an animal cell, a mammalian cell, a mouse cell, or a human cell. 
     
     
         27 . The isolated host cell of  claim 26 , wherein the host cell is a human cell. 
     
     
         28 . The virus of  claim 22 , wherein said virus is an adenovirus, adeno-associated virus or retrovirus. 
     
     
         29 . The vector of  claim 21 , wherein said vector is a plasmid. 
     
     
         30 . The vector of  claim 21 , wherein said vector is an expression vector. 
     
     
         31 . The vector of  claim 21 , wherein said vector is a viral vector. 
     
     
         32 . The isolated host cell of  claim 25 , wherein said cell is a mammalian cell. 
     
     
         33 . The isolated host cell of  claim 25 , wherein the host cell is selected from the group consisting of an  Aspergillus  cell, a  Trichoderma  cell, a  Saccharomyces  cell, a  Pichia  cell, a  Candida  cell, and a  Hansenula  cell. 
     
     
         34 . The isolated host cell of  claim 25 , wherein the host cell is selected from the group consisting of a  Syneechocystis  cell, a  Synechococcus  cell, a  Salmonella  cell, a  Bacillus  cell, an  Acinetobacter  cell, a  Rhodococcus  cell, a  Streptomyces  cell, an  Escherichia  cell, a  Pseudomonas  cell, a  Methylomonas  cell, a  Methylobacter  cell, an  Alcaligenes  cell, a  Synechocystis  cell, an  Anabaena  cell, a  Thiobacillus  cell, a  Methanobacterium  cell and a  Klebsiella  cell. 
     
     
         35 . The isolated host cell of  claim 25 , wherein the host cell is a plant cell. 
     
     
         36 . The isolated host cell of  claim 35 , wherein the plant cell is selected from the group consisting of an apple cell, an  Arabidopsis  cell, a bajra cell, a banana cell, a barley cell, a bean cell, a beet cell, a blackgram cell, a chickpea cell, a chili cell, a cucumber cell, an eggplant cell, a favabean cell, a maize cell, a melon cell, a millet cell, a mungbean cell, an oat cell, an okra cell, a  Panicum  cell, a papaya cell, a peanut cell, a pea cell, a pepper cell, a pigeonpea cell, a pineapple cell, a  Phaseolus  cell, a potato cell, a pumpkin cell, a rice cell, a sorghum cell, a soybean cell, a squash cell, a sugarcane cell, a sugarbeet cell, a sunflower cell, a sweet potato cell, a tea cell, a tomato cell, a tobacco cell, a watermelon cell, and a wheat cell.