Methods for in vitro maturation of ovarian follicles

Abstract

The invention relates to methods to be used in the maturation of ovarian follicles and oocytes. More specifically, the invention concerns the use of inhibitors of the phosphatase PTEN, such as oxovanadate and peroxovanadate complexes, in methods for in vitro and in vivo maturation of follicles and oocytes.

1 . (canceled) 
     
     
         2 . A method for the in vitro activation of mammalian non-growing follicles, the method comprising the step of incubating the mammalian follicles in a physiological acceptable medium comprising one or more inhibitors of PTEN. 
     
     
         3 . The method according to  claim 2 , wherein the non-growing follicles, are selected from primordial, intermediate and primary follicles. 
     
     
         4 . The method according to  claim 2 , wherein the activated follicles are further used for in vitro fertilization. 
     
     
         5 . The method according to  claim 2 , wherein the non-growing follicles are human. 
     
     
         6 . The method according to  claim 2  wherein the inhibitor of PTEN is selected from; 
       Bisperoxo(bipyridine)oxovanadate, 
       Bisperoxo(1,10-phenanthroline)oxovanadate, 
       Bisperoxo(picolinato)oxovanadate, 
       Bisperoxo(5-hydroxypyridine-2-carboxyl)oxovanadate, 
       Di-(picolinate) oxovanadate, 
       Di-(3-hydroxypicolinate) oxovanadate, 
       Bisperoxo(phenylbiguanide)oxovanadate, 
       Di-(phenylbiguanide)oxovanadate, or 
       Bisperoxo(isoquinolinecarboxylic acid)oxovanadate. 
     
     
         7 . A method for the in vitro maturation of an oocyte which comprises the activation of non-growing follicles according to  claim 2 . 
     
     
         8 . A method of in vitro fertilization comprising implanting an embryo, wherein said embryo is produced by a method comprising treating a mature oocyte with sperm, wherein said oocyte is produced by the method of  claim 7 .