Peptide and Complex of Same, Scaffold for Tissue Repair and Surface Treatment Method for Same, and Surface Treatment Solution or Set of Treatment Solutions

Abstract

Provided are a peptide that enables surface treatment of a scaffold for tissue repair that makes it possible to accelerate the repair of living tissue without using a material that negatively affects the repair of living tissue, a complex containing this peptide, a scaffold for tissue repair surface treated using this peptide or this complex, a surface treatment method for a scaffold for tissue repair using this peptide or this complex, and a treatment solution or set of treatment solutions to be used in this surface treatment method. Surface treatment of a scaffold for tissue repair is conducted by combining glycosaminoglycan and a peptide containing adhesive sites and basic sites each comprising predetermined amino acid residues.

1 . A peptide comprising, in the amino acid sequence thereof, an adhesive site consisting of residues of amino acids including a 2,3-dihydroxyphenyl group or 3,4-dihydroxyphenyl group, and a basic site including three or more consecutive basic amino acid residues. 
     
     
         2 . The peptide according to  claim 1 , wherein the adhesive site and the basic site bind via a spacer site. 
     
     
         3 . The peptide according to  claim 1 , wherein N terminus or C terminus is a basic terminus that includes a basic site at the terminus or vicinity thereof, and
 wherein a site in which an amino acid residue is not present, or consisting of at least 1 to no more than 5 amino acid residues and not including the adhesive site is present on a basic terminus side from the basic site.   
     
     
         4 . The peptide according to  claim 3 , wherein another end relative to the basic terminus is an adhesive terminus having an adhesive site at a terminus or vicinity thereof, and
 wherein the adhesive site is contained and the basic site is not contained within five amino acid residues from the adhesive terminus.   
     
     
         5 . The peptide according to  claim 4 , wherein a sequence of nine resides from the C terminus is an amino acid sequence represented by SEQ ID NO 1. 
     
     
         6 . A complex comprising the peptide according to  claim 1 , and glycosaminoglycan. 
     
     
         7 . A complex of the peptide according to  claim 1 , glycosaminoglycan and cell growth factor,
 wherein the cell growth factor is binding with the glycosaminoglycan, and binding with the adhesive site of the peptide via the glycosaminoglycan.   
     
     
         8 . A scaffold for tissue repair, wherein the peptide according to  claim 1  is binding to the surface thereof at the adhesive site. 
     
     
         9 . A scaffold for tissue repair having glycosaminoglycan on a surface thereof,
 wherein the peptide according to  claim 1  is binding with the surface of the scaffold at the adhesive site, and   wherein the glycosaminoglycan is binding with the basic site of the peptide.   
     
     
         10 . A scaffold for tissue repair having cell growth factor on a surface thereof,
 wherein the peptide according to  claim 1  is binding with the surface of the scaffold at the adhesive site, and   wherein the cell growth factor is binding with glycosaminoglycan, and binding with the basic site of the peptide via the glycosaminoglycan.   
     
     
         11 . (canceled) 
     
     
         12 . (canceled) 
     
     
         13 . (canceled) 
     
     
         14 . (canceled) 
     
     
         15 . (canceled) 
     
     
         16 . (canceled) 
     
     
         17 . A treatment solution for surface treatment of a scaffold for tissue repair, comprising the peptide according to  claim 1 . 
     
     
         18 . A set of treatment solutions for surface treatment of a scaffold for tissue repair, comprising the treatment solution according to  claim 17  and a treatment solution containing glycosaminoglycan. 
     
     
         19 . The set of treatment solutions for surface treatment of a scaffold for tissue repair according to  claim 18 , further comprising a treatment solution containing cell growth factor. 
     
     
         20 . A treatment solution for surface treatment of a scaffold for tissue repair, comprising the complex according to  claim 6 . 
     
     
         21 . A set of treatment solutions for surface treatment of a scaffold for tissue repair, comprising the treatment solution according to  claim 20  and a treatment solution containing cell growth factor. 
     
     
         22 . A treatment solution for surface treatment of a scaffold for tissue repair, comprising the complex according to  claim 7 .