Novel Flavivirus Antigens

Abstract

The invention provides polynucleotides and polypeptides encoded therefrom having advantageous properties, including an ability to induce an immune response to flaviviruses. The polypeptides and polynucleotides of the invention are useful in methods of inducing immune response against flaviviruses, including dengue viruses. Compositions and methods for utilizing polynucleotides and polypeptides of the invention are also provided.

1 . A recombinant or synthetic polypeptide comprising an amino acid sequence that has at least 95 percent amino acid sequence identity to the full-length sequence set forth in SEQ ID NO:139, 141, 142, 143, 144, 145, 146, 148, 236, 237, 343, or 345. 
     
     
         2 . The recombinant or synthetic polypeptide of  claim 1 , wherein the recombinant or synthetic polypeptide induces an immune response in a subject against at least one  flavivirus.    
     
     
         3 . The recombinant or synthetic polypeptide of  claim 1 , wherein the recombinant or synthetic polypeptide induces an immune response in a subject against at least one dengue virus. 
     
     
         4 . The recombinant or synthetic polypeptide of  claim 1 , wherein the recombinant or synthetic polypeptide induces an immune response in a mammal against dengue viruses of multiple serotypes. 
     
     
         5 . The recombinant or synthetic polypeptide of  claim 1 , wherein the recombinant or synthetic polypeptide comprises the full-length amino acid sequence set forth in SEQ ID NO:139, 141, 142, 143, 144, 145, 146, 148, 236, 237, 343, or 345. 
     
     
         6 . A composition comprising at least one recombinant or synthetic polypeptide and an excipient or carrier, wherein said polypeptide comprises an amino acid sequence that has at least 95 percent amino acid sequence identity to the full-length sequence set forth in SEQ ID NO:139, 141, 142, 143, 144, 145, 146, 148, 236, 237, 343, or 345. 
     
     
         7 . The composition of  claim 6 , wherein the recombinant or synthetic polypeptide induces an immune response in a subject against at least one  flavivirus.    
     
     
         8 . The composition of  claim 6 , wherein the recombinant or synthetic polypeptide induces an immune response in a subject against at least one dengue virus. 
     
     
         9 . The composition of  claim 6 , wherein the recombinant or synthetic polypeptide induces an immune response in a mammal against dengue viruses of multiple serotypes. 
     
     
         10 . The composition of  claim 6 , wherein the recombinant or synthetic polypeptide comprises the full-length amino acid sequence set forth in SEQ ID NO: 139, 141, 142, 143, 144, 145, 146, 148, 236, 237, 343, or 345. 
     
     
         11 . A virus-like particle comprising at least one polypeptide, wherein said polypeptide comprises an amino acid sequence that has at least 95 percent amino acid sequence identity to the full-length sequence set forth in SEQ ID NO:139, 141, 142, 143, 144, 145, 146, 148, 236, 237, 343, or 345. 
     
     
         12 . The virus-like particle of  claim 11 , wherein the recombinant or synthetic polypeptide induces an immune response in a subject against at least one  flavivirus.    
     
     
         13 . The virus-like particle of  claim 11 , wherein the recombinant or synthetic polypeptide induces an immune response in a subject against at least one dengue virus. 
     
     
         14 . The virus-like particle of  claim 11 , wherein the recombinant or synthetic polypeptide induces an immune response in a mammal against dengue viruses of multiple serotypes. 
     
     
         15 . The virus-like particle of  claim 11 , wherein the recombinant or synthetic polypeptide comprises the full-length amino acid sequence set forth in SEQ ID NO: 139, 141, 142, 143, 144, 145, 146, 148, 236, 237, 343, or 345.